部分缩窄大鼠腹主动脉致心肌肥厚动物模型的病证结合研究

目的:研究部分缩窄大鼠腹主动脉致心肌肥厚动物模型的中医证候特点及病理特点。方法:大鼠随机分为假手术组和模型组,采用部分缩窄大鼠腹主动脉致心肌肥厚。从观测术后每周动物一般状态以及术后4w、8w的心率、力竭性游泳时间、心电图、超声心动图等方面进行中医证候评价,并用超声心动图观测大鼠心脏的结构和功能变化。结果:术后4w与假手术组比较,模型组大鼠ST段发生异常变化(异常的抬高或降低)的导联数明显增加(P<0.01),心率明显增快(P<0.05),力竭性游泳时间明显缩短(P<0.01),室间隔舒张末期厚度(IVSTd)明显增加(P<0.01),左室舒张末期内径(LVDd)无明显变化,左室射血分数(EF)无明显变化,左室心肌质量指数(LVMI)明显增加(P<0.01);术后8w与假手术组比较,模型组大鼠ST段发生异常变化(异常的抬高或降低)的导联数仍明显增加(P<0.01),心率仍明显增快(P<0.05),力竭性游泳时间仍明显缩短(P<0.01),IVSTd继续非常显著增加(P<0.01),LVPWTd非常显著增加(P<0.01),LVDd显著增加(P<0.05),EF明显减小,LVMI非常显著性增大(P<0.01)。结论:部分缩窄腹主动脉致心肌肥厚大鼠心脏早期左心室呈现典型的向心性肥厚的病理改变。随着病变发展造模8w后,属于既有向心性肥厚又有离心性肥厚的混合型左室肥厚。在中医证候方面具有心气虚证及血瘀证的特点,血瘀证贯穿于病变的整个过程,而心气虚证是随着病变的发展逐渐出现和加重的。

Combination of Syndrome and Disease of Myocardial Hypertrophy Model Induced by Partial Coarctation Abdominal Aorta in Rats

Objective: To study the characteristic of syndromes and disease in Rat induced by Partial Coarctation Abdominal Aorta.Methods: Fifty-four Sprague Dawley rats were randomly assigned to sham(n=18) and model(n=36) groups.Myocardial hypertrophy was made with partial coarctation abdominal aorta.Observed general condition,heart rate,time of exhausted swimming,electrocardiogram,ultrasonograph in every week after operation.Identified syndromes of model groups on basis of standardizations of integrated traditional and western medicine academy about XuZheng and XueyuZheng.Results: Compared with sham groups,after 4 weeks,model rats had increased heart rate(P<0.05) and number of variation ST-section(P<0.01),but time of exhausted swimming(P<0.01) had shortened markedly.Interventricular septum thickness(IVSTd)(P<0.01) and left ventricular mass index(LVMI)(P<0.05) had increased significantly,however both left ventricle diameter(LVDd) and ejection fiction(EF) had no conspicuous difference.After 8 weeks,model rats still had increased heart rate(P<0.05) and number of variation ST-section(P<0.01),and had shortened the time of exhausted swimming(P<0.01).