| miR-581调控FOXO1对卵巢癌SKOV3细胞自噬的影响 |
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| 引用本文： | 高海宁,白瑞霞,赵鹏伟,宋婉莹,林萱.miR-581调控FOXO1对卵巢癌SKOV3细胞自噬的影响[J].肿瘤防治研究,2022,49(5):403-407. |
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| 作者姓名： | 高海宁 白瑞霞 赵鹏伟 宋婉莹 林萱 |
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| 作者单位： | 1. 010000 呼和浩特，内蒙古医科大学内蒙古临床医学院；2. 010000 呼和浩特，内蒙古自治区人民医院检验科；3. 010000 呼和浩特，内蒙古医科大学基础医学院；4. 137400 兴安盟，兴安盟人民医院检验科 |
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| 摘 要： | 目的 探讨miR-581对卵巢癌SKOV3细胞自噬的影响及作用机制。方法 将miR-581 mimics和miR-581 NC转染到卵巢癌SKOV3细胞中，实时荧光定量PCR法（qRT-PCR）检测转染效率；转染成功后，Western blot检测转染后卵巢癌SKOV3细胞中自噬相关蛋白的表达水平；TargetScanHuman数据库预测miR-581靶基因，Western blot验证miR-581与靶基因的作用。结果 过表达miR-581后可显著抑制自噬相关蛋白LC3Ⅱ和Beclin1的表达（P<0.01）；miR-581可负向调控FOXO1的表达（P<0.01）；FOXO1促进卵巢癌SKOV3细胞自噬（P<0.05）。结论 miR-581通过调控FOXO1的表达影响卵巢癌SKOV3细胞自噬。
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| 关 键 词： | 卵巢癌 SKOV3细胞 自噬 miR-581 FOXO1 |
| 收稿时间： | 2021-10-21 |
Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1 |
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| GAO Haining,BAI Ruixia,ZHAO Pengwei,SONG Wanying,LIN Xuan.Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1[J].Cancer Research on Prevention and Treatment,2022,49(5):403-407. |
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| Authors: | GAO Haining BAI Ruixia ZHAO Pengwei SONG Wanying LIN Xuan |
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| Institution: | 1. Inner Mongolia Medical College, Inner Mongolia Medical University, Hohhot 010000, China; 2. Department of Clinical Laboratory, Inner Mongolia People’s Hospital, Hohhot 010000, China; 3. Graduate School, Inner Mongolia Medical University, Hohhot 010000, China; 4. Department of Clinical Laboratory, Xing'anmeng People's Hospital, Xing'anmeng 137400, China |
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| Abstract: | Objective To explore the effect and mechanism of miR-581 on the autophagy of ovarian cancer SKOV3 cells. Methods miR-581 mimics and miR-581 NC were transfected into SKOV3 cells, and the transfection efficiency was detected by qRT-PCR. After successful transfection, Western blot was used to detect autophagy-related proteins expression in SKOV3 cells. TargetScanHuman database predicted miR-581 target genes, and Western blot verified the role of miR-581 and target genes. Results Overexpression of miR-581 significantly inhibited the expression of autophagy-related proteins LC3Ⅱ and Beclin1 (P<0.01); miR-581 negatively regulated FOXO1 expression (P<0.01) and FOXO1 promoted autophagy of SKOV3 cells (P<0.01). Conclusion miR-581 affects the autophagy of ovarian cancer SKOV3 cells by regulating the expression of FOXO1. |
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| Keywords: | Ovarian cancer SKOV3 cells Autophagy miR-581 FOXO1 |
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