Presence of δ-(l-α-Aminoadipyl)-l-Cysteinyl-d-Valine in Fermentations of Penicillium chrysogenum |
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Authors: | P Adriaens B Meesschaert W Wuyts H Vanderhaeghe H Eyssen |
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Institution: | The Rega Institute, University of Leuven, B-3000 Leuven, Belgium |
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Abstract: | Cultures of Penicillium chrysogenum, growth with 35S]sulfate or labeled amino acids, were examined by ion-exchange chromatography for possible peptidic precursors of penicillin. A sulfur-containing compound, present in both the mycelial extracts and the culture filtrates, was eluted at the location of the synthetic lld-tripeptide δ-(l-α-aminoadipyl)-l-cysteinyl-d-valine. Since this compound was also labeled when the cultures were incubated with dl-6-14C]α-aminoadipic acid, l-3,3′-3H]cystine, or dl-1-14C]valine, its identity with the synthetic lld-tripeptide can be accepted. No δ-(l-α-aminoadipyl)-l-cysteine or lll-tripeptide were detected. The implications of these findings for tripeptide and penicillin biosynthesis are discussed. |
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