一种基于momp基因的衣原体分子生物学甄别技术的初步建立

目的利用PCR方法建立一种基于momp基因的衣原体分子甄别方法。方法根据衣原体momp基因的恒定区和可变区分别设计衣原体科特异性引物和种特异性引物，利用PCR方法对本实验室保存的衣原体进行扩增，以达到甄别的目的。利用限制性片段长度多态性（RFLP）分析科特异性PCR扩增产物，研究momp基因的多态性。结果利用建立的PCR方法对本实验室保存的九株衣原体进行了研究，结果表明八株衣原体都是鹦鹉热嗜衣原体，一株为沙眼衣原体。利用限制性片段长度多态性（RFLP）分析科特异性PCR扩增产物，分析了D34、B11001、CW2和CP12四株衣原体，结果可以产生两种不同的带型。结论利用建立的PCR方法可以达到检测甄别衣原体的目的，并可以通过RFLP分析衣原体的侵袭性。

Preliminary establishment of a molecular method based on momp gene to differentiate chlamydiae

A molecular method based on the momp gene of chlamydiae was preliminarily established for the differentiation of this species , in which the genus and species specific primers were designed for PCR according to the constant and variable domains of the momp gene of chlamydiae, and these primers were used to amplify and differentiate chlamydiae studied.It was shown that these primers could successfully discriminate 9 strain of chlamydiae stored in our laboratory.Meanwhile, when the restriction fragment length polymorphism (RFLP) was used to differentiate chlamydiae by Alu I enzyme digestion, two different restriction patterns were demonstrated. These results illustrate that PCR and momp gene restriction mapping are the useful methods for the typing of chlamydiae