食管癌患者外周血清IL-8、IL-18水平的测定

目的了解食管癌患者手术前后白细胞介素-8（IL-8）、白细胞介素-18（IL-18）的水平及其与肿瘤临床分期之间的关系。方法用酶联免疫吸附试验（ELISA）检测130名对照者及80例食管癌早期（Ⅰ-Ⅱ）患者和58例晚期（Ⅲ-Ⅳ）患者手术前后血清IL-8、IL-18水平。结果食管癌早期组血清IL-8[（85.88±6.13）ng/L]、IL-18[（301.28±45.66）ng/L]和食管癌晚期组IL-8[（120.42±6.12）ng/L]、IL-18[（412.30±50.81）ng/L]均显著高于正常对照组IL-8[（54.38±11.18）ng/L]、IL-18[（181.36±50.32）ng/L,P〈0.01],晚期组较早期组升高（P〈0.01）;转移组手术后血清IL-8[（148.36±9.56）ng/L]、IL-18[（451.66±50.35）ng/L]水平较手术前IL-8[（122.94±9.85）ng/L]、IL-18[（415.35±51.05）ng/L]高（P〈0.01）,无转移组手术后IL-8[（71.68±9.66）ng/L]、IL-18[（225.99±41.78）ng/L]较术前IL-8[（85.86±9.70）ng/L]、IL-18[（311.58±40.35）ng/L]明显降低（P〈0.01）。结论食管癌患者存在IL-8和IL-18表达异常;IL-8、IL-18水平可作为食管癌临床分期、预后判断和病情监测的指标。

Detection of peripheral serum level of IL-8 and IL-18 in patients with esophageal cancer

Objective To study the serum level of interleukin-8 （IL-8） and interleukin-18 （IL-18） in patients with esophageal cancer before and after surgery, and the relationship between the levels and the clinic stages of cancer. Methods The serum levels of IL-8 and IL-18 were detected by enzyme-linked immunosorbent assay （ELISA）for 130 healthy subjects, 80 patients with early esophageal cancer（ Ⅰ -Ⅱ ） and 58 patients with late esophageal cancer（ Ⅲ-Ⅳ ）. Results The serum levels of IL-8 and IL-18 in patients with early esophageal cancer [ （85. 88 ± 6. 13 ） ng/L], [ （ 301.28 ± 45.66） ng/L] or late esophageal cancer [ （ 120.42 ± 6.12 ） ng/L ], [ （412.30 ± 50.81 ） ng/L] were much higher than in the control group [ （54.38 ± 11.18） ng/L], [ （ 181.36 ± 50.32） ng/L, P 〈 0.01 ], and the serum levels in the patierts with late esophageal cancer were much higher than in early esophageal cancer （ P 〈 0.01 ）. In the cancer metastasis group,the serum levels of IL-8 [ （ 148.36 ±9.56） ng/L] ,IL-18 [ （451.66 ±50.35） ng/L] in patients with esophageal cancer after surgery were higher than in the patients before surgery IL-8 [ （ 122.94 ± 9.85 ） ng/ L] ,IL-18[ （415.35 ±51.05） ng/L,P 〈0.01 ] ,and in the no-metastasis group, the serum levels of IL-8 [ （71.68 ±9.66 ng/L] ,IL-18 [ （225.99 ±41.78） ng/L] in patients with esophageal cancer after surgery were much lower than in the patients before surgery IL-8[ （85.86 ±9.70） ng/L] ,IL-18[ （311.58 ±40.35） ng/L,P 〈0. 01 ]. Conclusions The serum levels of IL-8 and IL-18 are abnormal in the patients with esophageal cancer, and the levels of IL-8 and IL- 18 might be an index for monitoring the clinical progress and disease condition,judging the prognosis of the cancer.