| Identification of Common Species of Dermatophytes by PCR-RFLP |
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| 引用本文： | 何甘霖 李家文 丁娟 谭志健. Identification of Common Species of Dermatophytes by PCR-RFLP[J]. 华中科技大学学报(医学英德文版), 2005, 25(4): 458-460. DOI: 10.1007/BF02828223 |
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| 作者姓名： | 何甘霖 李家文 丁娟 谭志健 |
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| 作者单位： | [1]Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong Universityof Science and Technology, Wuhan 430022, China |
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| 摘 要： | To establish a simple, sensitive and effective technique for the identification of six common dermatopbytes, polymerase chain reaction （PCR） and PCR-restriction fragment length polymorpbism （RFLP） targeting Topoisomerase Ⅱ gene were used. The DNA of 6 common dermatopbytes was amplified by primer dPsD1 and then primers dPsD2. The products generated by dPsD2 were digested with restriction enzyme Hinc Ⅱ and Hinf Ⅰ separately. A DNA fragment of about 3390 bp was amplified by using primer dPsD1 from the genomic DNA of each dermatopbyte species. The product of dPsD2 was 2380 bp and the restriction profiles of Hinc Ⅱ and Hinf Ⅰ were between 58-1670 bp. By using PCR-RFLP, all of the 6 dermatopbytoses were diagnosed to species level and no obvious difference identification between Hinc Ⅱ and Hinf Ⅰ. It is concluded that the PCR-RFLP identification of dermatopbytes by Hinc Ⅱ or Hinf Ⅰ is efficient and rapid in clinical practice.
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| 关 键 词： | 总核素 皮肤真菌 PCR RFLP 检查方法 |
| 收稿时间： | 2005-04-03 |
Identification of common species of dermatophytes by PCR-RFLP |
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| He Ganlin,Li Jiawen,Ding Juan,Tan Zhijan. Identification of common species of dermatophytes by PCR-RFLP[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2005, 25(4): 458-460. DOI: 10.1007/BF02828223 |
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| Authors: | He Ganlin Li Jiawen Ding Juan Tan Zhijan |
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| Affiliation: | Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | Summary To establish a simple, sensitive and effective technique for the identification of six common dermatophytes, polymerase chain reaction (PCR) and PCR0restriction fragment length polymorphism (RFLP) targeting Topoisomerase II gene were used. The DNA of 6 common dermatophytes was amplified by primer dPsD1 and then primers dPsD2. The products generated by dPsD2 were digested with restrictiion enzymeHinc II andHinf I separately. A DNA fragment of about 3390 bp was amplified by using primer dPsD1 from the genomic DNA of each dermatophyte species. The product of dPsD2 was 2380 bp and the restriction profiles ofHinc II andHinf I were between 58–1670 bp. By using PCR-RFLP, all of the 6 dermatophytoses were diagnosed to species level and no obvious difference identification betweenHinc II andHinf I. It is concluded that the PCR-RFLP identification of dermatophytes byHinc II orHinf I is efficient and rapid in clinical practice. HE Ganlin, male, born in 1977, Graduate Student |
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| Keywords: | dermatophyte PCR RFLP |
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