膀胱移行细胞癌的ClC-3和NF-κB的表达及意义

目的:探讨ClC-3和NF-κB在膀胱移行细胞癌中的表达及意义。方法:应用免疫组织化学染色技术检测31例病理诊断为膀胱移行细胞癌和6例正常膀胱组织中ClC-3和NF-κB的表达。结果:膀胱移行细胞癌组织中ClC-3主要在癌细胞的胞膜表达,阳性表达率74%(23/31)。其中膀胱移行细胞癌Ⅰ级阳性表达率40%(4/10),Ⅱ级阳性表达率81%(9/11)、Ⅲ级阳性表达率100%(10/10)。NF-κB在癌细胞的胞浆内表达,阳性表达率83%(26/31)。其中膀胱移行细胞癌Ⅰ级阳性表达率60%(6/10),Ⅱ级阳性表达率90%(10/11),Ⅲ级阳性表达率100%(10/10)。6例正常膀胱组织中仅有1例ClC-3表达弱阳性,NF-κB表达均为阴性。膀胱移行细胞癌组织中ClC-3和NF-κB阳性表达率高于正常膀胱组织(P<0.01)。结论:ClC-3和NF-κB可能与膀胱移行细胞癌病理分级和增殖有关。

Expressions of ClC-3 and NF-κB in transitional cell carcinoma of bladder and their significances

Objective To investigate the expressions of ClC-3 and NF-κB in transitional cell carcinoma of the bladder(TCCB) specimen and their significances. Methods The expressions of ClC-3 and NF-κB were detected in 31 cases of TCCB and 6 cases of normal bladder tissue as control by immunohistochemical staining.Results In the tissues of TCCB,ClC-3 was mainly located in the cell membrane,the positive rate of ClC-3 was 74%(23 /31);the posivive rates of Ⅰ,Ⅱ and Ⅲ grades were 40%(4/10),81%(9/11), and 100%(10/10),respectively.NF-κB was mainly distributed in the cytoplasm,the positive rate of NF-κB was 83%(26/31);the positive rates of Ⅰ,Ⅱ and Ⅲ grades were 60%(6/10),90%(10/11) and 100%(10/10),respectively.Only one specimen showed weak positive staining,and negative staining of NF-κB was found in 6 cases of normal bladder tissues.The positive rates of ClC-3 and NF-κB in tissue of TCCB were higher than those in normal bladder tissue(P<0.01).Conclusion ClC-3 and NF-κB may be related to the pathological grade and proliferation of TCCB.