胚胎兔、成兔皮肤在正常发育及创伤愈合过程中IgG的表达

目的 分析胚胎兔，成兔胚皮肤在便伤愈合过程中IgG表达的差异，为胎儿型及成人型愈合机制的研究提供依据。方法 在建立胎兔皮肤创伤模型的基础上，采用皮肤均化的方法，提取正常及创伤组织中的免疫球蛋白IgG，用双抗体夹心ELISA法进行含量测定。结果 随正常胎兔（孕23－28天）孕期的延长，皮肤中IgG有合成增加的趋势，而正常成免皮肤的IgG含量显著高于胎兔（P＜0．01）；胎兔皮肤创伤后不同时间（1－6d)的IgG含量与对应的正常胎兔（孕23－28天）相比，只伤后第1，2天增高（P＜0．01），而不同孕期的创伤胎兔之间差异无显著意义。成兔皮肤创伤后从第2天起IgG合成量明显高于正常成兔（P＜0．01），并呈现明显的上升趋势，对胎，成兔皮肤创伤后IgG变化趋势的动态发现胎兔呈平坦线性，成兔呈上升线性。结论 在胚胎发育成熟的过程中，免疫系统日臻完善，虽然IgG在较低水平，但在创伤后仍表现出一定的免疫反应，而成兔在创伤后却表现出强烈的免疫反应，不仅含量高而且趋势显著，免疫反应的不同是胎儿愈合与成人型愈合的本质差别之一。

IgG expressions of fetal and adult rabbit skin in normal development and wound healing

Objective To analyse the difference of IgG expressions between fetal and adult rabbit skin in wound healing for exploring the mechanisms of fetal-type and adult-type wound healing. Methods Based on a fetal rabbit wound healing model, we extracted IgG from normal and wounded tissues using the homogenization method and determined its value using ELISA technique in the comparative study. Results We found that the variation of IgG showed a tendency of elevation with the prolonging of pregnancy in normal rabbits(23-28 days gestation);the IgG value in adult rabbits was much higher than that of the fetuses(P＜0.01);IgG value in wounded fetuses was higher(P＜0.01) than that of corresponding normal fetuses during the first two days after wound creation, and there were no differences between wounded fetuses at different gestation times. From the 2nd day of wound creation, IgG value in wounded adults was much higher than the normal(P＜0.01) and had an obvious tendency of elevation. All the facts suggested that the immunological system became mature with the development of the fetus. Despite the lower IgG levels in the fetus,it also manifested some immunological reactions to injury whereas the adult took intense reactions with higher IgG value and an obvious tendency of elevation. Conclsion The difference of immunoreaction is one of the main reasons of the fetal-type and adult-type wound healin