IL-18基因启动子-607C/A位点多态性与川崎病的关系研究1

目的：探讨IL-18基因启动子-607C／A位点多态性与川崎病（KD）的关系。方法：收集50例KD患儿（KD组）和50例健康儿童（正常组）资料，采集血液标本，分离血浆和有核细胞，用SSP-PCR法检测IL-18基因-607位点基因型、酶联免疫吸附法测定血浆IL-18含量以及免疫比浊法测定血浆CRP含量。结果：KD组患儿-607C／A位CC基因型频率为42．0％，高于正常组儿童（Jp〈0．05）；CC基因型KD患儿血浆IL-18含量显著高于CA基因型患者（P〈0．01），前者血浆CRP含量也高于后者（P〈0．05）。结论：IL-18基因启动子-607C／A位点多态性参与KD发病，IL-18基因-607位点基因型的检测可用于预测KD发病和预后判断。

Studies on Relationship between-607 C/A Polymorphism of IL-18 Gene Promoter and Kawasaki Disease

Objective： To observe the relationship between-607 C/A polymorphism of IL-18 gene promoter and ka- wasaki disease（KD）. Methods： Fifty cases of children with KD （KD group） and 50 cases of health children（normal group） were collected and their blood specimens were taken and separated into two parts of plasma and nucleated cells.The geno- type of-607 C/A locus of IL-18 gene was determined by SSP-PCR, the content of IL-18 in plasma was measured by en- zyme-linked immunosorbent assay（ELISA）, and the level of CRP in plasma was detected by immune turbidimetry assay. Results： The frequency of CC genotype in -607 C/A locus of IL-18 gene for KD group children was 42.0%, more than that of normal group children（P〈0.05）. The plasma content of IL-18 for CC genotype KD children was notably higher than that for CA genotype KD children（P〈0.01）, and the plasma level of CRP of the fonner was also more than the latter （P〈0.05）. Conclusion： The polymorphisms of-607C/A locus in IL-18 gene can play a role in KD pathogenesis, and the test of the genotype of-607C/A locus in IL- 18 gene may be used to predict pathogenesis and to assess prognosis for the children with KD.