谷氨酸钠及r-氨基丁酸与大鼠尾壳核的铁代谢

BACKGROUND: Previous studies suggest that neurotransmitters can affect iron content, but the molecular mechanism is unclear. OBJECTIVE: To observe the regulation of Glutamate and GABA on iron metabolism in nigrostriatum of rats. DESIGN, TIME AND SETTING: Randomized grouping and immunohistochemistry study was performed at the institute of molecular neurobiology and neuropharmacology in Hebei normal university from July 2008 to October 2009. MATERALS: A total of 36 Male Sprague–Dawley rats were used to inject GMS or GABA into substantial nigra.METHODS: All the rats were divided into control group, GMS group and GABA group. Rats were anesthetized and positioned in a small-animal stereotaxic apparatus with a following injection of GMS and GABA. MAIN OUTCOME MEASURES: DMT1(-IRE) and HP content in substantial nigra and caudate putmanarea were studied by immunohistochemistry.RESULTS: The iron content in the caudate putamen nucleus of GMS group significantly increased, while iron content in GABA group decreased with no obvious change. At the meantime, the study showes that the expression of tyrosine hydroxylase (TH) in GMS group and GABA group shows no change. GMS significantly increase the level of DMT1(-IRE) while GABA decrease it. However, HP expression decreased in GMS group and increased in GABA group.CONCLUSION: Glutamate and GABA can affect iron metabolism through DMT1(-IRE) and HP in the nigrostriatum.

Sodium glutamate and gamma-aminobutyric acid affect iron metabolism in the rat caudate putamen

Glutamic acid and gamma-aminobutyric acid (GABA) influence iron content in the substantia nigra and globus pallidus, although the mechanisms of action remain unclear. The present study measured iron content and changes in divalent metal transporter 1 (DMT1) and hephaestin expression in the substantia nigra and caudate putamen, and explored the effects of GABA and glutamic acid on iron metabolism. Results demonstrated that iron content and DMT1 non iron response element [DMT1(-IRE)] expression were significantly greater but hephaestin expression was significantly lower in the caudate putamen of the monosodium glutamate group compared with the control group. No significant difference in iron content was detected between the GABA and control groups. DMT1(-IRE) expression was significantly reduced, but hephaestin expression was significantly increased in the GABA group compared with the control group. In addition, there was no significant difference in tyrosine hydroxylase expression between monosodium glutamate and GABA groups and the control group. These results suggested that glutamate affected iron metabolism in the caudate putamen by increasing DMT1(-IRE) and decreasing hephaestin expression. In addition, GABA decreased DMT1(-IRE) expression in the caudate putamen.