| 肛肠部沙眼衣原体感染的临床实验室研究 |
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| 引用本文： | 陈建江,陈国英,单平囡,卢志勇,谈素红,茹金城,许德顺. 肛肠部沙眼衣原体感染的临床实验室研究[J]. 中华医院感染学杂志, 2011, 0(17): 3743-3745 |
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| 作者姓名： | 陈建江 陈国英 单平囡 卢志勇 谈素红 茹金城 许德顺 |
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| 作者单位： | 1. 中国医科大学绍兴医院检验中心,浙江绍兴,312030
2. 绍兴文理学院附属医院肿瘤科,浙江绍兴,312000 |
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| 摘 要： | 目的探讨肛肠部沙眼衣原体(Ct)感染的实验室筛查和临床诊断,以提高临床检出率,减少误诊、误治的发生。方法采用涂片查包涵体、斑点免疫层析试验(DICA)和(或)PCR-荧光探针法,对53例疑似肛肠部Ct感染患者进行检测和临床分析。结果 53例肛肠部感染的分泌物Ct抗原阳性率为52.8%,总误诊率为75.0%;对2种采样方式选取的30例标本检测阳性率作一致性分析,两种采样方法的检测结果存在一致性(U=4.065,P<0.05);15例分泌物3种方法检测Ct,PCR与包涵体测定配对定性资料χ2检验分析,差异有统计学意义(P<0.05);PCR与免疫金标法测定配对,差异无统计学意义;免疫金标法较佳,包涵体法检出率最低。结论临床医师应重视肛肠部Ct感染的筛查和诊断,应用斑点免疫层析试验作检测是高效的选择,不要用染色涂片法检测包涵体检测肛肠部沙眼衣原体感染。
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| 关 键 词： | 沙眼衣原体感染 斑点免疫层析试验 聚合酶链反应 误诊 |
Chlamydia trachomatis causing anorectal infections:a clinical laboratory study |
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| CHEN Jian-jiang,CHEN Guo-ying,SHAN Ping-nan,LU Zhi-yong,TAN Su-hong,RU Jin-cheng,XU De-shun. Chlamydia trachomatis causing anorectal infections:a clinical laboratory study[J]. Chinese Journal of Nosocomiology, 2011, 0(17): 3743-3745 |
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| Authors: | CHEN Jian-jiang CHEN Guo-ying SHAN Ping-nan LU Zhi-yong TAN Su-hong RU Jin-cheng XU De-shun |
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| Affiliation: | CHEN Jian-jiang,CHEN Guo-ying,SHAN Ping-nan,LU Zhi-yong,TAN Su-hong,RU Jin-cheng,XU De-shun(Shaoxing Hospital,China Medical University,Shaoxing,Zhejiang 312030,China) |
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| Abstract: | OBJECTIVE To investigate the clinical diagnosis value of Chlamydia trachomatis to improve the clinical detection rate and reduce the misdiagnosis and mistreatment.METHODS The stain smear for inclusion body,dot immuno-chromatographic assay(DICA) and polymerase chain reaction(PCR) fluorescence probing were used for detecting Ct antigen and clinically analyzing 53 suspected patients with anorectal Ct infections.RESULTS It founded that 52.8% of Ct antigen-positive rate and 75.0% of total misdiagnosis rate in th... |
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| Keywords: | Chlamydia trachomatis infection Dot immuno-chromatographic assay Polymerase chain reaction Misdiagnosis |
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