胃癌组织中核不均一核糖核蛋白K的表达及其与幽门螺杆菌L型感染的关系

目的 探讨人胃癌组织中核不均一核糖核蛋白K(hnRNPK)的表达及其与幽门螺杆菌L型(Hp-L)感染的关系.方法 应用革兰氏染色和免疫组化法检测100例胃癌组织、50例癌旁组织及30例正常组织的Hp-L型感染情况,应用免疫组化Elivision法检测hnRNPK蛋白的表达,原位杂交方法检测hnRNPK mRNA的表达.结果 胃癌组、癌旁组和正常组Hp-L型感染的阳性率分别为67.0％、58.0％和23.3％,胃癌组与癌旁组差异无统计学意义(P＞0.05),但与正常组比较,差异有统计学意义(P＜0.05).胃癌组hnRNPK蛋白和mRNA的表达阳性率分别为82.0％和86.0％,均明显高于癌旁组和正常组(均P＜0.05).胃癌组织中hnRNPK蛋白的表达与胃癌的分化程度、淋巴结转移、临床分期有关(均P＜0.05).胃癌组Hp-L型感染阳性者的hnRNPK蛋白表达阳性率为92.5％,高于Hp-L型感染阴性组(60.6％,P＜0.05).相关分析表明,胃癌组hnRNPK蛋白表达与Hp-L型感染呈正相关(r=0.391,P＜0.01).结论 胃癌组织中hnRNPK高表达,Hp-L型感染可促进hnRNPK的表达,两者在胃癌发生、发展过程中可能具有协同作用.

Expression of hnRNPK in gastric carcinoma and its relationship with Helicobacter pylori L-form

Objective To investigate the expression feature of heterogeneous nuclear ribonucleoprotein Kin gastric carcinoma and its clinical significance,and to explore the relationship between hnRNPK expression and Helicobacter pylori L-form infection.Methods The expression of hnRNPK protein was examined in 100 cases of gastric carcinoma,50 paracancerous gastric tissues and 30 matched normal gastric mucosa by Elivision immunohistochemistry and hnRNPK-mRNA by in situ hybridization.Hp-L was detected with Gram staining and immunohistochemical staining.Results The positive rates of hnRNPK protein and mRNA in gastric carcinoma were 82.0％ and 86.0％,respectively,significantly higher than those in the paracancerous gastric tissues and normal controls(P ＜ 0.05).The expression of hnRNPK protein was significantly correlated with histological differentiation,TNM stage and lymph node metastasis (P ＜ 0.05).The positive rates of Hp-L in the three groups were 67.0％,58.0％ and 23.3％,respectively.The positive rate of Hp-L in gastric carcinoma had no significant correlation with it in the paracancerous gastric tissues,but was significantly higher than it in the normal controls(P ＜ 0.05).In gastric carcinoma,the expression of hnRNPK protein was higher in cases of Hp-L positive patients than those of Hp-L negative cases (P ＜ 0.05).Positive correlation existed between the expression of hnRNPK protein and Hp-L infection(r=0.391,P ＜ 0.01).Conclusions There is a higher expression of hnRNPK in gastric carcinoma.Hp-L infection may be associated with the up-regulated hnRNPK expression.The two factors may play a synergetic role in gastric carcinogenesis.