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Potassium assay in blastomere of two-cell mouse embryo after equilibration and washing from cryoprotector
Authors:D.?V.?Gol’dshtein,E.?I.?Smol’yaninova,A.?G.?Pogorelov  author-information"  >  author-information__contact u-icon-before"  >  mailto:pogorelov_a@mail.ru"   title="  pogorelov_a@mail.ru"   itemprop="  email"   data-track="  click"   data-track-action="  Email author"   data-track-label="  "  >Email author
Affiliation:(1) Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino
Abstract:The need for effective methods of cryopreservation of early mammalian embryos necessitates the study of the mechanisms of blastomere adaptation to the equilibration procedure and subsequent washing from the cryoprotector. The osmotic effects during these procedures can cause electrolyte imbalance in embryonic cells. Intracellular potassium concentrations at the stage of two-blastomere mouse embryo were studied by electron probe microanalysis. Translated fromByulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 138, No. 7, pp. 48–49, July, 2004
Keywords:cryopreservation  ethylene glycol  two-blastomere embryo  cytoplasmatic potassium  electron probe microanalysis
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