Quantitative localization of [3H]TCP binding in rat brain by light microscopy autoradiography |
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| Authors: | R Sircar S R Zukin |
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| Affiliation: | 1. Brain Imaging and Neurological Disability UMR 825, INSERM, F-31059 Toulouse, France;2. Brain imaging and neurological disability UMR 825, University of Toulouse, UPS, CHU Purpan, Place du Dr Baylac, F-31059 Toulouse Cedex 9, France;3. Research Center for Brain and Cognition, University of Toulouse UPS, Toulouse, France;4. CerCo, CNRS, Toulouse, France;5. Radiopharmacy Department, University Hospital, Toulouse, France;6. Animal experimentation platform, UMS 006, Toulouse, France;7. Research and Expertise Group, French Association for the Promotion of Medical Research (AFPREMED), Toulouse, France;8. Nuclear Medicine Department, University Hospital, Toulouse, France |
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| Abstract: | The anatomical localization of phencyclidine (PCP)/sigma-opiate receptors in rat brain was determined by quantitative light microscopy autoradiography using the new ligand N-(1-[2-thienyl]cyclohexyl) [3H]piperidine ([3H]TCP). TCP is a potent analog of PCP which possesses a higher affinity for PCP/sigma-opiate receptor than does PCP itself. The highest level of [3H]TCP binding was detected in the hippocampus. Intermediate levels were found in frontal cortex, striatum, amygdala and cerebellum. Specific [3H]TCP binding was undetectable in anterior commissure and corpus callosum. The distribution pattern of [3H]TCP binding sites is similar to the pattern obtained with [3H]PCP but more sharply defined. On the basis of its greater potency and specificity, [3H]TCP may prove superior to [3H]PCP as a molecular probe for the study of brain sigma opiate/phencyclidine receptors. |
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