|
|||||
|
|
| 地龙注射液HPLC指纹图谱的研究 | |
| 引用本文： | 詹云丽,黄璐敏,翁嘉,黄丹莹,李晓蒙,裘建社.地龙注射液HPLC指纹图谱的研究[J].广东药学院学报,2009,25(3):245-248. |
| 作者姓名： | 詹云丽 黄璐敏 翁嘉 黄丹莹 李晓蒙 裘建社 |
| 作者单位： | 1. 惠州市药品检验所,广东,惠州516003 2. 广东药学院,药科学院,广东,广州510006 3. 广东省博罗先锋药业集团有限公司,广东,惠州,516100 |
| 摘 要： | 目的建立地龙注射液中游离氨基酸的HPLC指纹图谱。方法采用2，4-二硝基氟苯柱前衍生化法，用HPLC法进行梯度洗脱分析地龙注射液中游离的氨基酸。结果通过优化衍生条件，得到分离度、重复性均较好的HPLC指纹图谱，确定了14个共有峰，以缬氨酸为参照峰，用中药指纹图谱相似度软件计算不同批次样品的相似度均在0．97以上。结论本研究建立的指纹图谱，可用于地龙注射液的质量控制。 |
| 关 键 词： | 地龙注射液 指纹图谱 HPLC 氨基酸 |
HPLC fingerprints of Dilong injection |
|
| ZHAN Yun-li,HUANG Lu-min,WENG Jia,HUANG Dan-ying,LI Xiao-meng,QIU Jian-she.HPLC fingerprints of Dilong injection[J].Academic Journal of Guangdong College of Pharmacy,2009,25(3):245-248. | |
| Authors: | ZHAN Yun-li HUANG Lu-min WENG Jia HUANG Dan-ying LI Xiao-meng QIU Jian-she |
| Institution: | ZHAN Yun-li1,HUANG Lu-min1,WENG Jia1,HUANG Dan-ying1,LI Xiao-meng2,QIU Jian-she3 (1.Guangdong Huizhou Institute for Drug Control,Huizhou,Guangdong 516003,China,2.Guangdong Pharmaceutical College,Guangzhou,Guangdong 510006,3.Guangdong Boluo Pioneer Medicinal Group Co.,LTD,Guangdong 516100,China) |
| Abstract: | Objective To establish the HPLC fingerprints of free amino acids in Dilong injection.Methods 1-fluoro-2,4-dinitrobenzene was used for the pre-column derivation of amino acids in the Dilong injection,then analyzed with acetonitrile-DMF-sodium acetate gradient elution.Results The HPLC fingerprints were well separated.Fourteen common peaks were obtained.The similarities among different batches of samples were all above 0.97 when using valine as reference peak.Conclusion This method could be used for the qualit... |
| Keywords: | HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |