环氧化酶-2、bcl-2与bax在胃癌的表达及意义

目的 研究环氧化酶-2(cox-2)与bcl-2、bax在胃癌中的表达及其与胃癌细胞凋亡的关系。方法 采用SP免疫组织化学法检测胃癌及癌旁组织各60例标本中cox-2、bcl-2、bax的表达，用脱氧核糖核酸末端转移酶介导的dUTP缺口末端标记法(TUNEL)检测胃癌及癌旁组织的凋亡率。结果 胃癌组织的cox-2、bcl-2的表达明显高于癌旁组织(P＜0．05)，bax的表达明显低于癌旁组织(P＜0．05)，cox-2阳性组胃癌组织的凋亡率显著低于阴性组；cox-2的表达与bcl-2呈正相关，与bax呈负相关。结论 cox-2、bcl-2、bax的异常表达与胃癌细胞凋亡抑制有关；cox-2能够上调bcl-2的表达并下调bax的表达使胃癌细胞凋亡抑制。

Expression and Significance of cyclooxygenase-2, bcl-2 and bax in Human Gastric Carcinoma

Objective To study the expression of cyclooxygenase\|2(cox\|2), bcl\|2 and bax gene in human gastric carcinoma tissues and the relationship between cox\|2, bcl\|2, bax and the apoptosis of gastric carcinoma cells. Methods S\|P immunohistochemistry was used to detect the expression of cox\|2, bcl\|2 and bax in 60 cases of gastric carcinoma and their adjacent noncancerous tissues, and terminal deoxynucleotidyl transferase\|mediated dUTP nick end labeling was used to detect the apoptosis in above tissues. Results The positive rates of cox\|2 and bcl\|2 in gastric carcinomas were significantly higher than those in the adjacent noncancerous tissues(P<0 05). The positive rate of bax in gastric carcinomas was significantly lower than the adjacent noncancerous tissues(P<0 05). The index of apoptosis in gastric carcinomas was significantly lower than the adjacent noncancerous tissues(P<0 05). Conclusion The apoptosis of gastric carcinoma cells correlated with the expression of cox\|2, bcl\|2 and bax. The high expression of cox\|2 up\|regulation the expression of bcl\|2 gene and down\|regulation of bax expression made the inhibition of apoptosis of gastric carcinoma cells.