CO2气腹对结直肠癌细胞侵袭转移能力的影响

目的探讨CO2气腹对结直肠癌细胞侵袭转移能力的影响。方法99例结直肠癌患者分为腹腔镜手术组50例和开腹手术组49例，通过免疫组织化学方法检测2组患者结直肠癌手术组织标本中缺氧诱导因子-1α、β-链蛋白和E-钙黏蛋白的表达，并进行比较。结果腹腔镜组与开腹组患者手术标本中缺氧诱导因子-1a（72％与67％）、β-链蛋白（38％与47％）和B钙黏蛋白（50％与49％）的表达率比较差异无统计学意义（P〉0．05）；腹腔镜组与开腹手术组p链蛋白（68％与65％）和E-钙黏蛋白（66％与57％）在细胞膜中表达缺失差异无统计学意义（P〉0．05）。结论CO2气腹不影响缺氧诱导因子-1α的表达及β链蛋白和E-钙黏蛋白的表达及结合，可能不促进结直肠癌的侵袭转移。

Influence of CO2 pneumoperitoneum on invasion capacity of colorectal cancer cells

Objective To investigate the influence of CO2 pneumoperitoneum on invasion and metastasis capacity of colorectal cancer cells. Methods A total of 99 cases of coloreetal cancer were divided into laparoscopy group （n=50） and open abdomincal operation group （n=49）. The expressions of hypoxia inducible factor-1 alpha （HIF-1α）, β-catenin and E-cadherin were detected with immunohistochemistry in two groups. Results There was no significant difference in the expressions of HIF-la （72% vs 67%）, β-catenin （38% vs 47%） and E-cadherin （50% vs 49%）, and in the loss expressions of β-catenin protein （68% vs 65%） and E-cadherin protein （66% vs 57%） between laparoscopy group and open abdomincal operation group （P〉0.05）. Conclusion CO2 pneumoperitoneum may not influence the expression of HIF-la, as well as the expression and binding of β-catenin and E-cadherin, and may not promote the invasion and metastasis of colorectal cancer.