大鼠弥漫性脑损伤后内耳听觉脑干反应和40Hz听觉相关电位及多频稳态反应与血红素加氧酶-1的相关性研究

目的探讨血红素加氧酶-1(heme oxygenase,HO-1)在弥漫性脑损伤(diffuse brain injury,DBI)大鼠耳蜗的表达,分析听觉脑干反应(auditory brainstem response,ABR)、4 0 Hz听觉相关电位(4 0 Hzauditory event related potential,4 0 Hz AERP)和多频稳态反应(auditory steady-state response,ASSR)与HO-1表达的相关性。方法建立DBI大鼠模型并随机分为5组,即正常对照组、外伤1、2、3、4周组,每组3 0只大鼠。采用ABR、4 0 Hz AERP、ASSR测定,光镜、免疫组织化学及扫描电镜观察各组动物HO-1表达及ABR、40Hz AERP、ASSR的变化。结果对照组大鼠耳蜗HO-1无表达,外伤后各组内耳HO-1表达有明显变化(P<0.01)。外伤后各组ABR、40Hz AERP、ASSR阈值之间比较有明显差别(P<0.05)。外伤各组内耳HO-1表达与ABR、40Hz AERP、ASSR阈值变化具有相关性(P<0.05)。结论大鼠DBI对内耳HO-1表达及听功能均有不同程度的影响,ABR、40 Hz AERP、ASSR阈值变化可能与HO-1表达有关。

Correlation study on the expression of HO-1 in cochlear with the changes of ABR, 40Hz AERP and ASSR in diffuse traumatic brain injury model rats

Objective To study the correlation between the expression of HO-1 in cochlear with the changes of auditory brainstem response （ABR）, 40Hz AERP and auditory steady-state response examination （ASSR） in diffuse traumatic brain injury model rats. Methods Diffuse traumatic brain injury model of rats were established and randomly divided into five groups, i.e. normal control, one week after trauma, 2, 3, and 4 weeks after trauma respectively. Then ABR, 40Hz AERP and ASSR, light microscope, electron microscope, immunohistochemistry technique were used to evaluate the changes of hearing function and morphology. Results The expression of HO-1 in the control group was normal, whereas there were obviously changes of HO- 1 expression in inner ears of the traumatic groups. The differences of thresholds of ABR, 40 Hz AERP and ASSR between the traumatic groups and the normal control group were obvious （ P 〈 0.05 ）. The expression of HO-1 in traumatic groups were significantly associated with the changes of auditory function （ P 〈 0.05 ）. Conclusion To varying degrees, the diffuse traumatic brain injury can influence the expression of HO-1 in the inner ear and auditory functions of model rats. The changes of thresholds of ABR, 40Hz AERP and ASSR may be associated with that of HO-1 expression in inner ear.