晚期氧化蛋白产物与大鼠椎间盘增龄性改变的关系

目的 研究大鼠在自然老化过程中椎间盘和血清中晚期氧化蛋白产物（AOPP）的变化。方法 60只SD大鼠分为青年组（3月龄）、成年组（9月龄）和老年组（22月龄）,每组20只,通过番红O/ 固绿染色观察大鼠椎间盘的组织学变化情况。测定血清及椎间盘组织匀浆中AOPP的含量。结果 各组椎间盘组织学均可见存在退变。三组60只大鼠椎间盘组织匀浆中AOPP的含量明显高于血清（t=6.66,P<0.01）,两者具有相关性（r=0.66,P<0.01）,血清及椎间盘组织中AOPP的含量随着年龄的增长而增加（F=19.65,P<0.01;F=42.04,P<0.01）,血清中AOPP含量老年组和成年组均明显高于青年组（P<0.01;P<0.01）,椎间盘组织均浆中AOPP含量老年组和成年组均明显高于青年组（P<0.01;P<0.01）结论 随着年龄的增长,椎间盘在老化的同时,AOPP的含量增加。AOPP与增龄性椎间盘变化存在重要关系。

The relationship between the change of Intervertebral Disc in aging and advanced oxidation protein products

Objective：To study the content change of advanced oxidation protein products in serum and intervertebral disc in aging. Method：60 SD male rats were divide into 3 group,younth group(3 months)、adult group(9months) and senile group(22months). The degeneration of the intervertebral disc observed by safranin-O and fast green stain.Result : The degeneration of the intervertebral disc were seen in all 3 groups.The concent of AOPP in intervertebral disc were obviously higher than that in serum（t=6.66,P<0.01）. The concent of AOPP in intervertebral disc was statistically correlated with that in serum（r=0.66,P<0.01）. The concent of AOPP in intervertebral disc and in serum were incresed with age（F=19.65,P<0.01;F=42.04,P<0.01）. The concent of AOPP in intervertebral disc in adult group and senile group were obviously higher than that in younth group（P<0.01;P<0.01）. And aslo in serum（P<0.01;P<0.01）. Conclusion: 　When the intervertebral disc degenerated with age, the content of AOPP were incresed.there is an important relationship between AOPP and the change of intervertebral disc.