大鼠纹状体边缘区与杏仁核和终纹床核之间相互联系的免疫组织化学研究

为了解大鼠纹状体内的神经活性物质与边缘系统重要结构杏仁核和终纹床核内相应神经活性物质之间的关系。对大鼠脑的连续冠状切片进行了 P物质、降钙素基因相关肽、亮氨酸 -脑啡肽、胆囊收缩素和神经元型一氧化氮合酶等的免疫细胞化学反应 ( ABC法 )。结果证明 :P物质、降钙素基因相关肽和胆囊收缩素在纹状体中主要分布在边缘区 ;亮氨酸 -脑啡肽主要分布在苍白球 ,其次分布在边缘区 ;神经元型一氧化氮合酶则主要分布在尾壳核和边缘区。上述神经活性物质同时也集中分布在杏仁核和终纹床核 ,而且边缘区内的这些神经活性物质与杏仁核和终纹床核内的相同物质存在着纤维联系。本研究结果说明 :边缘区与边缘系统之间存在着神经化学的相互关系 ,边缘区可能属于边缘系统的一部分。

THE CONNECTION AMONG THE MARGINAL DIVISION OF STRIATUM,AMYGDALOID NUCLEUS AND BED NUCLEUS OF THE STRIA TERMINALIS IN THE RAT BRAIN AS REVEALED BY IMMUNOHISTOCHEMISTRY

To understand the relationship of neurotransmitter between the striatum and limbic system such as amygdaloid nucleus and bed nucleus of the stria terminalis. 30 male Sprague Dawley rats were used. Immunohistochemical ABC method was performed to detect the expression of substance P (SP), calcitonin gene related peptide (CGRP), leucine enkephalin (L enk), cholecyctokinin (CCK) and neuronal nitric oxide synthase (NOS) on seetions of the brain. Some transmitters including substance P, calcitonin gene related peptide and cholecyctokinin were mainly distributed at the marginal division of the striatum. The leucine enkephalin was mainly distributed at the globus pallidus and was secondly distributed at the marginal division of the striatum. The neuronal nitric oxide synthase was mainly distributed at caudate putamen and the marginal division. All these transmitters were not only distributed at amygdaloid nucleus and bed nucleus of the stria terminalis, but also had fibers connection among the amygdaloid nucleus, marginal division and bed nucleus of the stria terminalis. CONCLUSION: There were special fibers connection between the marginal division and other basal ganglia nucleus or the limbic system. The marginal division may be played some important functions of basal ganglia and limbic system.