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Corticotropin-releasing factor (CRF) inhibits gonadotropin-releasing hormone (GnRH) release from superfused rat hypothalami in vitro
Authors:K.E. Nikolarakis   O.F.X. Almeida  A. Herz
Affiliation:1. School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China;2. School of Material Science and Engineering, Jiangsu Key Laboratory of Material Surface Technology, Changzhou University, Changzhou 213164, China;3. Qualtec Co., Ltd, Osaka 590-0906, Japan;1. Centro de Enseñanza Práctica e Investigación en Producción y Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Cruz Blanca 486 San Miguel Topilejo, Deleg. Tlalpan, CP 14500 Ciudad de México, Mexico;2. Instituto Tecnológico de Mérida, Ave. Tecnológico Km 5, Carretera Mérida-Progreso, CP 97118 Mérida, Yucatán, Mexico;3. Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a La Victoria km 0.6, CP 83304 Hermosillo, Sonora, Mexico;4. Universidad Autónoma Metropolitana-Unidad Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, Deleg. Iztapalapa, CP 09340 Ciudad de México, Mexico;5. U.S. Meat Export Federation, Jaime Balmes No. 8, Piso 6, Despacho 602-C, Col. Los Morales Polanco, Deleg. Miguel Hidalgo, CP 11510 Ciudad de México, Mexico
Abstract:A dose-dependent suppression of basal gonadotropin-releasing hormone (GnRH) release was observed when rat hypothalamic slices were superfused with human/rat corticotropin-releasing factor (CRF) (10(-12) to 10(-8) M). CRF was also found to significantly reduce the amount of GnRH released in response to 56 mM KCl. These in vitro results demonstrate that the CRF inhibition of GnRH secretion observed in vivo occurs within the hypothalamus and independently of the CRF activation of the pituitary-adrenal-gonadal axis.
Keywords:corticotropin-releasing factor (CRF)   gonadotropin releasing hormone (GnRH)   luteinizing hormone-releasing hormone (LHRH)   hypothalamus
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