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| 异种角膜基质异位移植对大鼠外周血T细胞亚群的影响 | |
| 引用本文： | 罗小玲,徐锦堂,江振友,吴静,赵松滨,陈建苏.异种角膜基质异位移植对大鼠外周血T细胞亚群的影响[J].中国病理生理杂志,2004,20(4):613-615. |
| 作者姓名： | 罗小玲 徐锦堂 江振友 吴静 赵松滨 陈建苏 |
| 作者单位： | 1. 暨南大学医学院第二附属医院深圳市人民医院眼科, 广东 深圳 518020; 2. 暨南大学医学院眼科教研室, 广东 广州 510632; 3. 暨南大学医学院微生物学与免疫教研室, 广东 广州 510632 |
| 基金项目： | 广东省重点科学基金资助项目 (No .A30 2 0 2 0 10 2 )，深圳市科技局基金资助项目 (No .2 0 0 2 0 4 0 30 ) |
| 摘 要： | 目的: 比较3种异种角膜基质的免疫原性。方法: 将SD大鼠36只随机分为4组,每组9只。1组为对照组,2-4组为实验组。分别获取新鲜猪、兔、鸡角膜基质,等量湿重25 mg异位植入大鼠背部皮下,术后观察伤口愈合情况,并于术后7 d、14 d、28 d获取大鼠外周血,免疫荧光标记及流式细胞仪分析比较3种异种角膜基质对大鼠CD4+、CD8+、CD25+、CD71+表型的动态影响。结果: 各组植入处皮肤无红肿、无渗出及其它改变,伤口愈合良好;各组与对照组比较:猪角膜基质组各时相表达的CD4+、CD8+、CD25+、CD71+T细胞比例无显著差异(P＞0.05);兔角膜基质组术后7 d,CD4+T细胞升高(P＜0.05),鸡角膜基质组术后7 d,CD4+、CD4+CD71+升高(P＜0.01)。结论: 3种异种角膜基质对大鼠细胞免疫原性比较:鸡>兔>猪,猪角膜基质的免疫原性最低。 |
| 关 键 词： | 异种角膜基质 移植 受体 白细胞介素2 CD71 流式细胞术 |
| 文章编号： | 1000-4718(2004)04-0613-03 |
| 收稿时间： | 2003-09-15 |
Effect of heteroge nic corneal stroma transplantation on peripheral T cells of rats |
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| LUO Xiao-ling,XU Jin-tang,JIANG Zhen-you,WU Jing,ZHAO Song-bin,CHEN Jian-su.Effect of heteroge nic corneal stroma transplantation on peripheral T cells of rats[J].Chinese Journal of Pathophysiology,2004,20(4):613-615. | |
| Authors: | LUO Xiao-ling XU Jin-tang JIANG Zhen-you WU Jing ZHAO Song-bin CHEN Jian-su |
| Institution: | 1. Department of Ophthalmology, Shenzhen People's Hospital, The Second Affiliated Hospital, Medical College of Jinan University, Shenzhen 518020, China; 2. Department of Ophthalmology, Medical College of Jinan University, Guangzhou 510632, China; 3. Department of Microorganism and Immunity, Medical College of Jinan University, Guangzhou 510632, China |
| Abstract: | AIM: To Compare immunogenicity of three kinds of heterogenic corneal stroma. METHODS: 36 SD rats were randomized into 4 groups, each group consisting of 9 rats. Group 1 was control group. Three kinds of heterogenic corneal stroma: porcine, rabbit and chicken corneal stroma were heterotopically transplanted to subcutaneous layer of 27 (group 2-4) SD rats, respectively. The expression of CD4 , CD8 , CD25 , CD71 on peripheral T cells was identified and analyzed by dual fluorescence flow cytometry at 7, 14, 28 days after operation. RESULTS: Compared with control group, the expression of CD4 , CD8 , CD25 , CD71 was no significant change in porcine corneal stroma group(P>0.05), the expression of CD4 was increased in rabbit corneal stroma (P<0.05), CD4 , CD4 CD71 markedly higher in the chicken corneal stroma (P<0.01) at 7 days after operation. CONCLUSION: The immunogenicity of porcine stroma is the lowest in three kinds of heterogenic corneal stroma (chicken, rabbit and porcine). |
| Keywords: | Heterogenic corneal stroma Transplantation Receptors interlenkin-2 CD71 Flow cytometry |
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