非小细胞肺癌组织中MT蛋白与PCNA表达的研究

目的:研究金属硫蛋白(metallothionein, MT)和增殖细胞核抗原(proliferatingcellnuclearan tigen,PCNA)在非小细胞肺癌(non smallcelllung carcinoma,NSCLC)中的表达及其与临床病理因素 的关系。方法:选取NSCLC组织62例(鳞癌38 例,腺癌24例)及正常肺支气管黏膜组织15例石 蜡标本,用SP法检测MT及PCNA在各组织中的表 达。结果:MT蛋白和PCNA在NSCLC中的阳性表 达率分别为53.2%(33/62)和79.0%(49/62),均 明显高于正常肺支气管黏膜组织[20.0%(3/15)和 46.7%(7/15)],差异有统计学意义,P<0.05。肺 鳞癌和腺癌中MT的阳性表达率分别为50.0% (19/38)和58.3%(14/24);PCNA的阳性表达率分 别为76.3%(29/38)和83.3(20/24),其差异均无 统计学意义。MT蛋白和PCNA的阳性表达率均与 NSCLC组织的分化程度、淋巴结转移等因素密切相 关,差异有统计学意义,P<0.05。MT与PCNA阳 性表达呈正相关,P=0.004。结论:MT蛋白的高 表达可能是NSCLC发生的一种生物学标志,且与 PCNA存在协同作用。联合检测两种蛋白的表达水 平对判断NSCLC恶性程度、淋巴转移趋势具有重要 价值。

Expressions of MT and PCNA in non-small cell lung carcinoma

OBJECTIVE:To investigate the expressions of metallothionein (MT) and proliferating cell nuclear antigen (PCNA) in non-small cell lung carcinoma. METHODS: The expressions of MT and PCNA were observed by immunohistochemical methods in 62 cases of non-small cell lung carcinoma and 15 cases of non-lung cancer tissue. RESULTS: The positive rates for MT and PCNA in NSCLC were 53.2%(33/62)and 79.0%(49/62) respectively which were significantly higher than these in non-lung cancer tissue, 20.0%(3/15)and 46.7%(7/15), P<0.05. There was no significant difference between the expressions of MT and PCNA in lung squamous cell carcinoma 50.0%(19/38)and 76.3%(29/38)and lung adenocarcinoma 58.3%(14/24) and 83.3(20/24). The positive rates of MT and PCNA were correlated with histological grades and lymph node metastasis. The differences were significant, P<0.05. The positive rates between MT and PCNA were associated, P=0.004. CONCLUSIONS: MT over expression may be a biological marker in non-small cell lung carcinoma. It was associated with the expression of PCNA. The levels of MT and PCNA were useful molecular markers for evaluating malignancy degree and lymph node metastasis of NSCLC.