内皮抑素和avastin对小鼠脉络膜新生血管抑制作用的比较观察

目的：观察内皮抑素（恩度）对C57BL/6J小鼠脉络膜新生血管的抑制效应，并与avastin作比较。方法：采用532nm激光光凝建立小鼠脉络膜新生血管模型，2wk后进行病理组织学检查，观察CNV的形成情况。取40只成功造模的小鼠，随机分成空白对照组（1组，10只）、生理盐水组（2组，10只）、内皮抑素组（3组，10只）和avastin组（4组，10只），光凝后2wk玻璃体腔给药1次，1wk后取材行HE、免疫组化染色观察结果。统计分析采用SPSS 16.0，方法为方差分析，多个样本两两比较用LSD-t检验，取P〈0.05作为检验水准。结果：2wk后HE病理组织学检查，光镜下可见大量新生血管形成，CNV阳性检出率为72.8%。生理盐水组与对照组比较，P〉0.05，对CNV无抑制作用； 内皮抑素组与空白对照组比较，P〈0.05，具有一定的抑制效应； avastin组与空白对照组比较，P〈0.05，对CNV有明显的抑制效应； 对avastin和内皮抑素组进行LSD-t检验，得出P〈0.05，差别有统计学意义，可以认为两者抑制CNV的效应是不相等的，由于（-overx）avastin =26.90，（-overx）内皮抑素=29.13，（-overx）avastin〈（-overx）内皮抑素，我们可推测，内皮抑素的抗CNV作用弱于avastin。结论：激光诱导有色小鼠C57BL/6J的CNV动物模型，成模时间短，成模率高，是进行CNV研究的理想模型。内皮抑素对CNV具有一定的抑制作用，未来很有可能成为眼科临床抗击CNV相关疾病的重要药物。

Comparison of endostatin(endostar)and avastin's inhibition effect on mice choroidal neovascularization

AIM:To observe the inhibition effect of endostatin(endostar)on mice choroidal neovascularization model(CNV)and compare with the Avastin.

METHODS: Using 532nm laser photocoagulation to establish a mouse model of CNV. We observed the formation of CNV by histopathological examination after 2wk later. Forty successful models of mice were randomly divided into control group(group 1, 10 rats), normal saline group(group 2, 10 rats), endostatin group(group 3, 10 rats)and avastin group(group 4, 10 rats). The drugs were injected into the mice' vitreous after photocoagulation 2wk later. Then 1wk later, we took the mice eyeballs to perform the HE and immunohistochemical staining to observe. The statistical analysis of ANOVA was done by SPSS 16.0 and the LSD-t test was used for multiple samples, taking P<0.05 as the test standards.

RESULTS: Two weeks later, HE histopathological examination was done, light microscope showed large amount of new vessels' formation, the positive rate for CNV was 72.8%. The blank control group compared with the normal saline group P>0.05, had no inhibitory effect on CNV; endostatin treated group compared with control group, P<0.05, had a certain inhibitory effect; avastin group compared with the control group, P<0.05, had an inhibitory effect on CNV; the LSD-t was performed on Avastin group and endostatin group, P<0.05, which were statistically significant. We thought that the two drugs have different inhibitory effect on mice' CNV, because (-overx)_Avastin =26.90,(-overx)_endostatin=29.13,(-overx)_Avastin<(-overx)_endostatin, we can infer that endostar had lower inhibitory effect on mice CNV than Avastin.

CONCLUSION: Laser-induced CNV animal models of colored mice C57BL/6J is of short time and high rate establishment and it is an ideal model for CNV study. Endostar has certain inhibitory effect on CNV, and it is likely to become one of the important drugs for CNV-related diseases in the future.