Coamplification of the L-myc and N-myc Oncogenes in a Neuroblastoma Cell Line |
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| Authors: | Takeshi Jinbo Yukio Iwamura Michio Kaneko Shigenori Sawaguchi |
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| Affiliation: | Department of Pediatric Surgery, Institute of Clinical Medicine, Institute of Basic Medical Science, University of Tsukuba, 1-1-1, Tennoudai, Tsukuba-shi, Ibaraki 305;Department of Microbiology, Institute of Basic Medical Science, University of Tsukuba, 1-1-1, Tennoudai, Tsukuba-shi, Ibaraki 305 |
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| Abstract: | The L- myc , N- myc and c- myc genes are members of the myc oncogene family. In particular, L- myc is novel, and amplification of L- tnyc is still unknown except in small cell lung carcinoma. We examined L- myc amplification in 30 human neuroblastomas using Southern blot hybridization, and found that the L- myc gene was amplified approximately 5-fold in GOTO, a human neuroblastoma cell line. The N- myc gene was also amplified approximately 60-fold and furthermore, over-expression of L- myc and N- myc genes was observed in this cell line. In this report, we describe the coampliflcation of the myc gene family in the GOTO neuroblastoma cell line. |
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| Keywords: | Coamplification L-myc N-myc Neuroblastoma |
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