中空羟基磷灰石复合rhBMP-2修复骨缺损过程的再血管化研究

目的　探讨和观察中空羟基磷灰石复合rhBMP-2在骨缺损修复过程的再血管化。  方法　将48只成年的新西兰雄性大白兔制作成桡骨骨缺损模型，随机分3组，各组分别植入以下材料：中空HA/ rhBMP-2复合人工骨、单纯中空HA人工骨、单纯rhBMP-2。植入后于4、8、12、16周分别注射99mTc-MDP进行放射性核素骨显像并监测骨缺损修复过程中再血管化情况，同时进行大体、X线、组织学观察。  结果　术后各时间段，中空HA/ rhBMP-2复合人工骨组在X线及放射性核素聚集强度明显高于单纯中空HA人工骨组(P<0.05) ，表现为成骨代谢活跃及早期的再血管化能力。  结论　中空HA/ rhBMP-2复合人工骨具有良好的骨缺损修复能力，成骨活性持久，再血管化能力强，有望成为一种理想的骨缺损修复材料。

Revascularization study of the hollow hydroxyapatite composite rhBMP-2 in repairing the bone defect

Objective　To study and observe the revascularization process of the hollow hydroxyapatite composite rhBMP-2 in repairing the bone defect.　Methods　48 adult male New Zealand rabbits with artificially-established radius defect were randomly divided into 3 groups, each group were implanted with the following materials: hollow HA/ rhBMP-2 composite artificial bone(A groups), hollow HA(B groups),rhBMP-2(C groups). After implantation in 4、8、12、16 weeks, the process was  observed using radionuclide bone imaging, X ray, histological observation.　Results　Different time after the operation, X-ray and histological score, bone radionuclide-gathering strength of the rabbits in the hollow HA/ rhBMP-2 composite artificial bone group in the were higher than that of hollow HA artificial bone (P＜0.05). In the control group, synostosis and bone sclerosis were observed in bone defect area bone defect failed to heal. Conclusion　Hollow HA/ rhBMP-2 composite artificial bone has good ability in revascularization and repairing segmental bone defect and is expected to be an ideal material for bone defect repair.