| 消胀方对四氯化碳诱导大鼠肝纤维化的防治作用 |
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| 引用本文： | 孙学华,张鑫,周振华,李曼,金树根,何林蔓,高亚婷,高月求.消胀方对四氯化碳诱导大鼠肝纤维化的防治作用[J].中国实验方剂学杂志,2015,21(9):102-107. |
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| 作者姓名： | 孙学华 张鑫 周振华 李曼 金树根 何林蔓 高亚婷 高月求 |
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| 作者单位： | 上海中医药大学 附属曙光医院, 上海市中医临床重点实验室, 上海 201203,上海中医药大学 附属曙光医院, 上海市中医临床重点实验室, 上海 201203,上海中医药大学 附属曙光医院, 上海市中医临床重点实验室, 上海 201203,上海中医药大学 附属曙光医院, 上海市中医临床重点实验室, 上海 201203,上海中医药大学 附属曙光医院, 上海市中医临床重点实验室, 上海 201203,上海中医药大学 附属曙光医院, 上海市中医临床重点实验室, 上海 201203,上海中医药大学 附属曙光医院, 上海市中医临床重点实验室, 上海 201203,上海中医药大学 附属曙光医院, 上海市中医临床重点实验室, 上海 201203 |
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| 基金项目： | 国家“重大新药创制”科技重大专项(2012ZX10005004-002,2012ZX-10005010-002-003);国家自然科学基金项目(81102570) |
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| 摘 要： | 目的:观察消胀方对四氯化碳(CCl4)诱导的大鼠肝纤维化的防治作用。方法:Wistar雄性大鼠66只随机分为6组,分别为正常组、CCl4模型组、秋水仙碱组、消胀方低、中、高剂量组,除正常组外,均采用CCl4诱导大鼠成肝纤维化模型,造模开始时分别给予秋水仙碱1 mg·kg-1,消胀方200,400,800 mg·kg-1ig 7周。观察大鼠体重和肝脾指数的变化;采用HE染色和天狼猩染色观察肝组织病理及胶原沉积情况;检测血清丙氨酸氨基转移酶(ALT),门冬氨酸氨基转移酶(AST),血清透明质酸(HA),层黏蛋白(LN),Ⅲ型前胶原(PCⅢ);检测肝组织羟脯氨酸(Hyp),超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px),丙二醛(MDA)含量。结果:与正常组比较,模型组大鼠的体重明显下降(P0.05),大鼠肝脏指数和脾脏指数均明显增高(P0.01),大鼠血清ALT,AST,HA,LN,PCⅢ水平明显升高(P0.01),大鼠肝组织Hyp,MDA含量明显升高(P0.01),SOD和GSH-Px活性明显降低(P0.01);与模型组比较,秋水仙碱和消胀方组ALT,AST,HA,LN,PCⅢ均明显下降(P0.01,P0.05),Hyp,MDA含量明显下降(P0.01,P0.05),SOD和GSH-Px活性明显升高(P0.01,P0.05);肝组织HE染色显示模型组大鼠肝脏肝细胞明显变性坏死,肝小叶结构破坏,假小叶形成,肝间质大量炎性细胞浸润;秋水仙碱和消胀方组肝组织假小叶数目减少,炎性细胞浸润减少;天狼猩红染色显示模型组大鼠肝窦周围胶原沉积明显;秋水仙碱和消胀方组胶原纤维明显减少。结论:消胀方对CCl4诱导的大鼠肝纤维化有一定的防治作用,其机制可能与保护肝细胞、减轻肝脏炎症和抗脂质过氧化损伤有关。
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| 关 键 词： | 肝纤维化 四氯化碳 消胀方 羟脯氨酸 脂质过氧化 |
| 收稿时间： | 2014/8/20 0:00:00 |
Effect of Xiaozhang Recipe on Liver Fibrosis Induced by Carbon Tetrachloride in Rats |
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| SUN Xue-hu,ZHANG Xin,ZHOU Zhen-hu,LI Man,JIN Shu-gen,HE Lin-man,GAO Ya-ting and GAO Yue-qiu.Effect of Xiaozhang Recipe on Liver Fibrosis Induced by Carbon Tetrachloride in Rats[J].China Journal of Experimental Traditional Medical Formulae,2015,21(9):102-107. |
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| Authors: | SUN Xue-hu ZHANG Xin ZHOU Zhen-hu LI Man JIN Shu-gen HE Lin-man GAO Ya-ting and GAO Yue-qiu |
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| Institution: | Shuguang Hospital Affiliated Shanghai University of Traditional Chinese Medicine, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai 201203, China,Shuguang Hospital Affiliated Shanghai University of Traditional Chinese Medicine, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai 201203, China,Shuguang Hospital Affiliated Shanghai University of Traditional Chinese Medicine, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai 201203, China,Shuguang Hospital Affiliated Shanghai University of Traditional Chinese Medicine, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai 201203, China,Shuguang Hospital Affiliated Shanghai University of Traditional Chinese Medicine, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai 201203, China,Shuguang Hospital Affiliated Shanghai University of Traditional Chinese Medicine, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai 201203, China,Shuguang Hospital Affiliated Shanghai University of Traditional Chinese Medicine, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai 201203, China and Shuguang Hospital Affiliated Shanghai University of Traditional Chinese Medicine, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai 201203, China |
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| Abstract: | |
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| Keywords: | liver fibrosis carbon tetrachloride Xiaozhang recipe hydroxyproline lipid peroxidation |
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