左、右归丸对去卵巢骨质疏松症大鼠模型骨密度及骨代谢的影响

目的:观察左、右归丸对去卵巢骨质疏松模型大鼠骨密度及骨代谢的影响。方法:通过切除6月龄SPF级SD雌性大鼠双侧卵巢的方法建立绝经后骨质疏松症大鼠模型。造模10周后,将80只模型大鼠随机分为左归丸高、中、低剂量组(n=10),右归丸高、中、低剂量组(n=10),阳性药组(n=10),模型组(n=10),造模时只切除腹腔内部分脂肪组织而未切除卵巢的实验大鼠设为假手术组(n=10),并分别ig给予相应药物或蒸馏水,各给药剂量分别为左、右归丸高剂量组20 g·kg-1·d-1;左、右归丸中剂量组10 g·kg-1·d-1;左、右归丸低剂量组5 g·kg-1·d-1;阳性药组为戊酸雌二醇片0.1 mg·kg-1·d-1;假手术组和模型组ig 10 m L·kg-1·d-1生理盐水。干预10周后,检测实验大鼠股骨骨密度(BMD)、骨组织形态学改变,采用酶联免疫吸附测定法(ELISA)测定大鼠血清骨碱性磷酸酶(BALP)、骨钙素(OC)、骨保护素(OPG)的含量。结果:与假手术组相比较,模型组大鼠BMD,OPG显著降低,而BALP,OC显著升高(P0.05),其病理改变与绝经后骨质疏松症患者改变一致;与模型组相比较,左、右归丸干预组大鼠BMD增加,骨组织形态改善,血清OPG含量升高、血清BALP,OC含量降低(P0.05)。结论:左、右归丸能够抑制骨吸收,纠正骨代谢失衡对绝经后骨质疏松症具有一定的治疗作用。

Effects of Zuogui Wan and Yougui Wan on Bone Mineral Density and Bone Metabolism of Ovariectomized Rats with Osteoporosis

Objective: To observe the effect of Zuogui Wan and Yougui Wan on bone mineral density and bone metabolism of ovariectomized rats with osteoporosis. Method: Post menopausal osteoporosis(PMOP) rats model was established by excision bilateral ovaries of 6 month old SD female rat.After successful modeling,80 modeling rats were randomly divided into model group, estradiol valerate group,Zuogui Wan groups with different doses,and Yougui Wan groups with different doses. Rats with resection of abdominal adipose tissue were divided into sham group. The changes of bone mineral density(BMD), serum bone alkaline phesphatase(BALP), serum osteocalcin(OC) and serum osteoprotegerin(OPG) deoxypyridinoline were detected respectively after 10 weeks. Result: Compared to sham operation group,BMD and serum OPG levelin of model group rats were decreased,but serum BALP, OC were increased. Compared with the model group, BMD, serum OPG of rats in Yougui Wan groups and Zuogui Wan groups were improved and serum BALP, OC content were decreased. Conclusion: Zuogui Wan and Yougui Wan can inhibit bone resorption, correct bone metabolism imbalance, which is beneficial to treatment of post menopausal osteoporosis