神经激肽-1受体拮抗剂WIN62577对哮喘气道重塑大鼠气管平滑肌细胞迁移的影响

目的 探讨哮喘气道重塑大鼠中气管平滑肌细胞迁移的变化及神经激肽-1受体(NK-1R)拮抗剂WIN62577对其的影响。方法 将Sprague-Dawley(SD)大鼠随机分为对照组和哮喘气道重塑组,应用卵清蛋白对模型组大鼠激发8周,原代培养纯化气管平滑肌细胞(ASMC),通过免疫荧光、实时定量PCR等方法,检测ASMC中NK-1R的表达,并给予不同剂量WIN62577干预,应用transwell小室检测ASMC迁移的变化。结果 哮喘气道重塑组ASMC中NK-1R主要分布在细胞质和细胞膜,其mRNA的表达高于对照组(PP-11 mol/L、10-10 mol/L、10-9 mol/L、10-8 mol/L)的WIN62577干预后ASMC迁移数目较哮喘气道重塑组明显减少(P结论 NK-1R能通过促进ASMC迁移能力而影响哮喘气道重塑。

Effects of NK-1R inhibitor WIN62577 on the migration of airway smooth muscle cells in asthmatic rats with airway remodeling

Objective To study the changes in the migration of airway smooth muscle cells (ASMC) in asthmatic rats with airway remodeling and the effect of NK-1R inhibitor WIN62577 on the migration of ASMC. Methods Sprague-Dawley rats were randomly assigned into two groups: airway remodeling induced by asthma and normal control. ASMC from rats with asthma and airway remodeling induced by ovalbumin (OVA) inhalation for 8 weeks were primary cultured and purified. Immunofluorescence and real-time PCR were used to measure the expression of NK-1R. With NK-1R inhibitor WIN62577 treatment, the changes in the migration of ASMC were measured by transwell chambers. Results NK-1R in ASMC was expressed mainly in the cytoplasm and cell membrane in the airway remodeling group, and the mRNA expression of NK-1R was higher than the normal control group (P<0.01). The number of the migrated ASMC in the airway remodeling group was significantly higher than that in the normal control group (P<0.01). Various concentrations (10^-11 mol/L, 10^-10 mol/L, 10^-9 mol/L and 10^-8 mol/L) of WIN62577 treatment decreased the number of the migrated ASMC (P<0.05). Conclusions NK-1R may affect airway remodeling possibly through promoting the migration ability of ASMC in rats with asthma.