新型多层平板型生物人工肝治疗急性肝功能衰竭犬的疗效

目的 评价新型多层平板型生物人工肝治疗急性肝功能衰竭动物的疗效.方法 以新鲜猪肝细胞及猪骨髓基质干细胞为细胞来源,共培养于新型多层平板型生物反应器内,从而构建一种新型的生物人工肝.采用D-氨基半乳糖给药方式构建犬急性肝功能衰竭模型,实验组(n=8)给予生物人工肝治疗;对照组(n=8)仅给予一般监护.观察和检测所有动物一般情况、生化指标及生存率.结果 实验组动物经生物人工肝治疗后,肝性脑病及一般精神状况均得到较明显改善,丙氨酸氨基转移酶从( 1512±183) U/L降至(86±25) U/L;天冬氨酸氨基转移酶从(1472±365) U/L降至(46±1l)U/L;乳酸脱氢酶从(463±76) U/L降至(312±84)U/L;总胆红素从(28.8±6.2) μmol/L降至(12.5±3.6) μmol/L;血氨从(56±15)μmol/L降至(34±10) μmol/L,同时凝血功能及白蛋白水平亦得到改善.8条犬中,5条存活、3条死亡,治疗过程中未出现严重并发症.对照组动物一般情况未见明显改善,各项化验指标呈逐渐加重趋势,最终8条犬中5条死亡,3条存活.但两组生存率的差异无统计学意义(P=0.294).结论 新型多层平板型生物人工肝对急性肝功能衰竭动物具有显著疗效,是治疗急性肝功能衰竭的一种有效支持手段.

The efficacy research of multi-layer flat-plate bioartificial liver on acute liver failure

Objective To evaluate the efficacy of newly developed multi-layer flat-plate bioartificial liver in treatment of canines with acute liver failure.Methods Porcine hepatocytes and bone marrow mesenchymal stem cells were cocultured in newly developed multi-layer flat-plate bioreactor.Acute liver failure in canine models was induced by D-galactosamine administration.Sixteen canine models were divided into two groups:treatment group ( n =8 ) and control group ( n =8 ).Biochemical parameters were determined for 7 days after treatment and liver specimens were collected for histological analysis.Results Hepatic encephalopathy and general conditions were significantly improved in the treatment group,but no changes in the control group.Alanine aminotransferase was significantly decreased from ( 1512 ± 183 )U/L to ( 86 ± 25 ) U/L in the treatment group,aspartate aminotransferase was significantly decreased from ( 1472 ± 365) U/L to (46 ± 11 )U/L,lactate dehydrogenase was significantly decreased from (463 ± 76 )U/L to (312 ±84)U/L,total bilirubin was significantly decreased from ( 28.8 ± 6.2 ) μmol/L to ( 12.5 ± 3.6)μmol/L,ammonia was significantly decreased from (56 ± 15 ) μmol/L to (34 ± 10) μmol/L,and prothrombin time were significantly decreased in the treatment group but increased in the control group,albumin was improved in the treatment group but decreased in the control group.There were 5 canines survived in the treatment group but only 3 in the control group.But there was no difference on survival rates between the two group( P =0.294).Conclusion The application of newly developed multi-layer flat-plate bioartificial liver system was effective in the treatment of canines with acute liver failure.