紫草素对IL-17刺激HaCaT细胞分泌VEGF影响的研究

目的 观察白细胞介素-17(IL-17)对HaCaT细胞分泌血管内皮生长因子(VEGF)的影响及紫草素的干预作用。方法 收集IL-17刺激组和紫草素处理后HaCaT细胞及其培养土清,分别用双抗体夹心酶联免疫吸附法(ELISA)及实时荧光免疫定量-聚合酶链反应法(RT-PCR)检测VEGF含量。应用细胞计数盒-8(CCK-8)检测各组HaCaT细胞活力。结果 与对照组相比,不同时间IL-17刺激组HaCaT细胞及其培养上清中VEGF表达均高于对照组(P＜0.001);紫草素处理组HaCaT细胞及其培养上清VEGF表达均低于IL-17处理组(P＜0.001);与对照组比较,CCK-8检测各组HaCaT细胞活力无明显差异(P＞0.05)。结论 IL-17可促进HaCaT细胞分泌VEGF,呈时间依赖型,而紫草素对其具有抑制作用。

The research of that Shikonin effects on VEGF production in IL-17-stimulated HaCaT cells

Objective To investigate whether IL-17 could stimulate the vascular endothelial growth factor (VEGF) production on HaCaT cells alone. We also investigated whether shikonin could inhibited the proinflamation effects of interleukin-17(IL-17) acting on HaCaT cells. Methods We examined the expression of VEGF by double antibody sandwich enzyme-linked immunosorbent assay ( ELISA ) and realtime polymerase chain reaction(RT-PCR) in HaCaT cells and the cell supernatant. The viability of HaCaT cells in the drug group was detected by the Cell Counting Kit-8 (CCK-8). Results The expression of VEGF in different time IL-17-stimulated groups on HaCaT cells and the cell supernatant were higher than the control group( P＜0.001 ). The expression of VEGF in different drug treatment groups on HaCaT cells and the cell supematant were lower than the stimulated group by IL-17 ( P＜0. 001 ). The cell viability of different drug treatment groups have no significant difference( P＞0.05 ). Conclusion We show that IL-17 specifically and time-dependently augmented and induced VEGF expression on HaCaT cells and the cell supernatant Then shikonin markedly inhibited the increase tengency of IL-17 effection on HaCaT cells and the cell supematant level.