小鼠受γ射线全身照射后FL表达变化的研究

目的 FL(fit3 ligand)是一种作用于早期造血细胞的生长因子，为进一步研究FL水平与辐射所致造血损伤的相关性，观察了小鼠受γ射线全身照射后FL的表达变化。方法以^60Coγ射线全身一次性均匀照射所致骨髓型急性放射病小鼠为模型，应用ELISA、流式细胞术、Western blot法检测照射前后小鼠外周血、骨髓、胸腺与脾脏中FL的表达变化。结果 小鼠受2～10Gyγ射线照射后24h，血清中FL浓度即有增加，并且随时间推移FL水平逐渐升高；吸收剂量在2～6Gy之间时，FL的水平随剂量增加而增加。6Gyγ射线照射后24h，外周血白细胞膜表面FL的表达增加，但24h后细胞膜表面FL的表达没有升高。6Gyγ射线照射后24h，骨髓、胸腺和脾脏有核细胞膜表面FL的表达明显升高，总蛋白中FL的水平亦明显增加。结论 辐射后早期小鼠FL可溶性与膜结合型蛋白的水平都有升高，而且辐射后血清中FL的水平与受照剂量及照射后时间存在相关性。

Research on change of FL expression in mice irradiated with gamma rays

Objective FL is a growth factor acting on hematopoietic progenitors.To further study the relationship between FL level and hematopoietic injury caused by radiation,the expression of FL in mice after whole body irradiation was observed. Methods C57BL/6J mice were irradiated with 2 Gy to 10 Gy of 60Co gamma rays.The expression level of FL in blood,bone,marrow,thymus and spleen of mice was quantified by methods of ELISA,FACS and Western blot. Results (1)Following irradiation with 2 Gy to 10 Gy,mice showed a gradual increase of serum FL level beginning from the first day after irradiation.At the doses from 2 Gy to 6 Gy,the increase of serum FL level in mice after irradiation was correlated to radiation dose;(2)At the first day after 6 Gy irradiation,membranebound FL on white blood cells in the peripheral blood of mice increased slightly,but on the following days it was not higher in the irradiated mice than that in the normal controls;(3)The membranebound FL level in bone marrow,thyums and spleen of mice increased on the first day after 6 Gy irradiation,so did the FL level in protein extraced from these three tissuesas measured by Western blot. Conclusion The expression level of soluble and membranebound FL in mice was significantly increased at the early stage following irradiation,and the level of serum FL was correlated with radiation dose and the time after irradiation.;