肝癌细胞株中肿瘤特异性抗原GAGE基因mRNA的表达 |
| |
引用本文: | 赵飞兰,蓝玲,罗国容,谢小薰. 肝癌细胞株中肿瘤特异性抗原GAGE基因mRNA的表达[J]. 华夏医学, 2006, 19(3): 392-393 |
| |
作者姓名: | 赵飞兰 蓝玲 罗国容 谢小薰 |
| |
作者单位: | 广西中医学院组胚教研室,广西,南宁,530001;广西医科大学组胚教研室,广西,南宁,530021 |
| |
基金项目: | 国家自然科学基金资助(No.30360026) |
| |
摘 要: | 目的:研究肿瘤特异性抗原GAGE基因mRNA在BEL-7404肝癌细胞株中的表达情况,评价其作为分子肿瘤标记以及肿瘤免疫治疗特异性靶位的可能性。方法:运用RT-PCR技术检测国内建株的肝癌细胞株BEL-7404中GAGE基因mRNA的表达,并与正常肝组织比较。结果:肝癌细胞株BEL-7404中GAGE基因呈阳性表达,正常肝组织中GAGE基因表达阴性。结论:GAGE基因可作为肝癌诊断的分子标记,并具有作为肝癌免疫治疗特异性靶位的潜在价值。
|
关 键 词: | BEL-7404肝癌细胞株 RT-PCR技术 GAGE基因 |
文章编号: | 1008-2409(2006)03-0392-02 |
修稿时间: | 2005-12-20 |
The mRNA expression of cancer-specific antigen GAGE gene in hepatocellular carcinoma cell line |
| |
Abstract: | Objective:To investigate the mRNA expression of cancer antigen GAGE gene in hepatocellular carcinoma(HCC) cell line.Methods: With Reverse Transcripase Polymerase Chain Reaction(RT-PCR),BEL-7404 HCC cell line was tested and was compared with normal liver tissue.Results: GAGE gene mRNA was expressed in BEL-7404 cell line but negative expression in normal liver tissue.Conclusion: The tumor antigen of GAGE can be used as molecular markers for early diagnosis and possible targets for immunotherapy of human HCC. |
| |
Keywords: | BEL-7404 cell line RT-PCR GAGE Gene |
本文献已被 CNKI 维普 万方数据 等数据库收录! |