醋酸甲孕酮对雄性大鼠骨髓嗜多染红细胞和生精细胞微核的影响

背景与目的:观察单独使用不同剂量的醋酸甲孕酮(Meddroxy progesterone acetate,MPA)对雄性大鼠骨髓和生精细胞微核的影响.材料与方法:50只大鼠分组后,给予每月一次MPA(37.5或75或150 mg/kg)肌肉注射;另以等体积生理盐水为对照,共3个月,每组中的一半动物在停药后即处死,另一半动物在停药9个月后处死.测定各组大鼠给药后骨髓与生精细胞微核率和精子计数及形态.结果:与对照组相比,各实验组大鼠生精功能均明显受到抑制.各实验组大鼠骨髓嗜多染红细胞的微核发生率无明显升高.各实验组生精细胞微核率明显增加,同时附睾精子观察不仅精子数量下降,而且畸形率升高,其中大剂量MPA组生精细胞微核率增加和精子畸形率升高最为明显;但停药9个月后各实验组生精细胞微核率下降至空白对照水平,同时附睾精子数量增加,畸形率减低.结论:单独使用MPA对雄性大鼠体细胞无致突变效应;虽然可以引起生精细胞微核率明显增加,但停药9个月后恢复.

The Micronucleus Test of Meddroxyprogesterone Acetate in Bone Marroww Polychromatic Erythrocytes and Spermatogenic Cells of Malerats

BACKGROUND&AIM: To study the effect of Meddroxyprogesterone Acetate on the micronucleus rate in bone marrowpolychromatic erythrocytes and spermatogenic cells of male rats. MATERIAL AND METHODS: 50rats were classified into4groups.Each group received injection of saline(groupC)or MPA(37.5,75and150mg/kg)(group P 1 ,group P 2 and group P 3 ,respectively)for every month during3months.Data from sperm counting,sperm modality,and the micronucleus rate in bone marrow polychromatic erythrocytes and spermatogenic cells were collected and analysed. RESULTS: Spermatogenesis of rats undergoing administration of MPA had been suppressed.Testis of rats in group P 1 ,P 2 and P 3 atrophied and sperm counting decreased remarkably compared with group C.There was no statistics difference of the micronucleus rate in bone marrow polychromatic erythrocytes between each group.The micronucleus rate in spermatogenic cells in group P 2 and P 3 raised,but resumed to normal after MPA deprived9months. CONCLUSION: MPA has no significant effecton increasing the incidence of the micronucleus rate in bone marrow polychromatic erythrocytes,while it can reversibly raise the micronucleus rate in spermatogenic cells.