培养的人动脉平滑肌细胞诱导高密度脂蛋白氧化修饰

目的　探讨高密度脂蛋白氧化修饰发生的机制。方法　利用体外培养的人动脉平滑肌细胞与人血浆高密度脂蛋白共同温育后 ,用琼脂糖凝胶电泳方法观察高密度脂蛋白电泳迁移率 ,紫外光分光光度法测定高密度脂蛋白共轭二烯的含量 ,利用荧光分光光度法测定其硫代巴比妥酸反应物质的量 ,用可见光分光光度法测定高密度脂蛋白的脂氢过氧化物含量。结果　人血浆高密度脂蛋白与人动脉平滑肌细胞共同培养 4 8h后 ,与天然高密度脂蛋白比较 ,其电泳迁移率明显增加 ,其共轭二烯、硫代巴比妥酸反应物质及脂氢过氧化物的含量均比天然高密度脂蛋白显著增加 (P <0 .0 1)。结论　体外培养人动脉平滑肌细胞能使高密度脂蛋白发生氧化修饰

Oxidative Modification of High Density Lipoprotein Induced by Cultured Human Arterial Smooth Muscle Cell

Aim To observe the mechanism of oxidative modification of high density lipoprotein induced by cultured human arterial smooth muscle cell. Methods The oxidative modification of high density lipoprotein was identified by agarose gel electrophoresis mobility change, absorbance at 234 nm by ultraviolet light, level of lipid hydroperoxide by visible light and fluorescence of thiobarbuturic acid reaction substance of human plasma high density lipoprotein. Results The electrophoretic mobility of high density lipoprotein was increased and absorbance at 234 nm, lipid hydroperoxide and thiobarbuturic acid reaction substance of high density lipoprotein incubated by cultured human arterial smooth muscle cell were significantly higher than that of the native high density lipoprotein. Conclusion Oxidative modification of high density lipoprotein could be induced by cultured human arterial smooth muscle cell.