电针内关穴对急性心肌缺血家兔心肌组织能量代谢的实验研究

目的:通过急性心肌缺血家兔内关穴电针的实验研究,观察针刺内关穴对心肌缺血家兔心肌能量代谢的影响,探讨经穴和脏腑相关联性。方法:将健康新西兰家兔40只随机分为正常组、盐水组、模型组、单侧针刺组和双侧针刺组5个组,采用经耳缘静脉持续长时间滴注垂体后叶注射液制成家兔急性心肌缺血模型,给予单侧和双侧内关穴电针,取心肌组织制成病理冰冻切片,通过染色,用电子显微镜观察糖原、琥珀酸脱氢酶和三磷酸腺苷酶各组的形态学变化。结果:①心肌缺血家兔心肌糖原、琥珀酸脱氢酶、ATP酶有耗竭、脱失现象(P0.05);②电针内关穴能增加心肌组织糖原、琥珀酸脱氢酶和三磷酸腺苷酶的活性,增强能量代谢,改善心肌缺血;③双侧针刺组比单侧针刺组的治疗效果明显(P0.05)。结论:①心肌缺血时,能量代谢相关酶发生改变;针刺内关穴,心肌缺血得到改善,心肌相关酶的异常变化得以纠正。②针刺内关穴能增强能量代谢物质的活性。③说明经脉上的理化刺激对相应脏腑功能有调节作用。

The Experimental Study of Effectiveness on Energy Metabolism of Cardiac Muscle of Acute Heart Muscle Ischemia Rabbits by Means of" Neiguan" Electroacupuncture

Objective：To observe the effectiveness on energy metabolism of cardiac muscle through the experimental study on acute heart muscle ischemia rabbits by means of ＂Neiguan＂ electro-acupuncture.And To discuss the relationship between acupuncture point and internal organs.Methods：40 healthy New Zealand rabbits were selected into 5 parts of the research： normal group,sodium chloride group,model group,one side acupuncture group and two sides acupuncture group,duplicate heart ischemia model on rabbits by means of PPI auditive vein injection continuously.Take tissue of cardiac muscle to observe the change of energy metabolism under electron microscope.Results：1.After PPI auditive vein injection on rabbits,the gray scale numerical value of glycogen,SDH and ATP compared with acupuncture group were decreased under microscope（P0.05）,after acupuncture the gray scale numerical value were increased by comparison with model group（P0.05）.2.The therapeutic efficacy of two sides acupuncture group is more obvious than that of one side acupuncture group.Conclusion：1.Enzyme of energy metabolism changed when it bccame heart muscle ischemia.The condition of heart muscle ischemia were improved and the abnormality change of heart muscle enzyme were retrieved when electro-acupuncture on ＂Neiguan＂.2.The material activity of energy metabolism were reinforced by electro-acupuncture on ＂Neiguan＂.3.The stimulation on meridians had regulative effects on the corresponding zang-fu function.