多潘立酮在增强结肠运动中的作用

目的 观察多潘立酮对结肠的促动力作用,并与莫沙必利及西沙必利比较.方法 ①在体实验:将40只大鼠分为对照组、多潘立酮组、莫沙必利组和西沙必利组,每组10只.在各组大鼠近端结肠和远端结肠埋植应力传感器,记录清醒大鼠结肠运动.②离体实验:在恒温灌流肌槽中,采用张力传感器测定多巴胺和多巴胺+多潘立酮对大鼠离体结肠肌条的收缩活动.结果 在体实验:①清醒大鼠在消化间期的静息状态下,结肠呈现节律性相位收缩活动.②多潘立酮可明显增加结肠的收缩活动,使近端结肠和远端结肠平均振幅分别比对照组增加84.61%±7.26%和76.37%±8.47%,呈剂量-效应关系.同等剂量的莫沙必利对近端结肠和远端结肠平均振幅分别比对照组增加50.32%±8.16%和45.13%±7.16%.莫沙必利对结肠的促动力作用明显低于多潘立酮.而同等剂量的西沙必利对近端结肠和远端结肠平均振幅分别比对照组增加92.55%±8.37%和81.27%±9.95%,其促动力作用与多潘立酮相同.离体实验:①灌流多巴胺(40 mg/ml)可明显抑制离体结肠肌条收缩活动,较Krebs-Ringer液对照组减少91.56%±10.24%.②多潘立酮可阻断多巴胺对离体结肠肌条的舒张作用.结论 多潘立酮可明显增强结肠动力,其作用明显优于莫沙必利,并与西沙必利作用相同.多潘立酮增强结肠动力作用是通过抗多巴胺作用实现.

Effect of domperidone on enhancement of colonic motor activity

Objective To observe the prokinetic effect of domperidone on colon and compare with that of mosapride and cisapride.Methods ① In viva experiment:forty rats were divided into control,domperidone,mosapride,and cisapride groups with 10 in each group.Stran gauges were planted both in proximal and distal colons and colonic motor activities were recoded in conscious rats.② In vitro experiment:the prokinetics effects of dopamine or dopamine combined with domperidone on the contraction of isolated rat colon strips were recorded by tone-transducers in the thermostatic muscle bath.Results In viva experiments:① in the interdigestive period of resting,activities of rhythimic phasic contraction were recoded in colon at conscious rats,and ② it had been showed that dornperidone significantly enhanced the contraction of colon in dose-dependent manner.Compared with control group,the mean contraction amplitude of proximal colon and distal colon increased by 84.61% ± 7.26% and 76.37 % ± 8.47% in domeridone group,respectively,which was higher than those in mosapride group (50.32%±8.16% and 45.13%±7.16%,respectively),but as same as those in cisapride group (92.55% ± 8.37% and 81.27% ± 9.95%,respectively).In vitro experiments:① perfusion of dopamin (40 mg/ml) could significantly decrease the contraction of isolated colon strip by 91.56% ± 10.24% in comparison with Krebs-Ringer solution,and ① domperidone could block the relaxant effect of dopamin on the isolated colon strip.Conclusions Domperidone can significantly enhance the colonic motor activities via antidopaminergic action.The effects of domperidone are similar as eisapride and greater than mosapride.