| 3个X染色体短串联重复的复合扩散及其多态性 |
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| 引用本文： | 刘秋玲,吕德坚,崔崴.3个X染色体短串联重复的复合扩散及其多态性[J].中华医学遗传学杂志,2004,21(3):233-235. |
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| 作者姓名： | 刘秋玲 吕德坚 崔崴 |
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| 作者单位： | 刘秋玲（510089,广州,中山大学中山医学院法医物证教研室）
吕德坚（510089,广州,中山大学中山医学院法医物证教研室）
崔崴（510089,广州,中山大学中山医学院法医物证教研室） |
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| 摘 要： | 目的 建立3个X染色体特异性短串联重复(X-chromosome specific shortt andem repeat，X-STR)基因座的复合扩增体系，并调查其多态性。方法 复合扩增DXS6799、DXS6804和DXS68543个基因座。并用聚丙烯酰胺凝胶电泳和银染法显色技术进行基因分型。结果 在广东汉族262名无关男性个体及255名无关女性个体中，3个基因座均发现了7个等位基因，男性个体共检出73种单倍型，单倍型多样性为0．9674。结论 这3个基因座的复合扩增系统有较高的多态性信息，在个体识别和亲权鉴定中有潜在的应用价值。
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| 关 键 词： | X染色体 短串联重复 复合扩散 多态性 |
| 修稿时间： | 2003年6月7日 |
Polymorphism and multiplex amplification of 3 X-chromosome specific short tandem repeat loci |
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| Qiu-ling Liu,De-jian Lü,Wei Cui.Polymorphism and multiplex amplification of 3 X-chromosome specific short tandem repeat loci[J].Chinese Journal of Medical Genetics,2004,21(3):233-235. |
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| Authors: | Qiu-ling Liu De-jian Lü Wei Cui |
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| Institution: | Faculty of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou, Guangdong, 510089 PR China. qiulinguser@tom.com |
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| Abstract: | OBJECTIVE: To devise a multiplex PCR system of three X-chromosome specific short tandem repeat (X-STR) loci and study the genetic polymorphism. METHODS: DXS6799, DXS6804 and DXS6854 were amplified simultaneously using a multiplex system and were typed by polyacrylamide gel electrophoresis and silver staining. RESULTS: A total of 262 male and 255 female individuals from Guangdong Han population were tested; each locus showed 7 alleles. 73 haplotypes were detected in the male individuals. The haplotype diversity reached 0.9674. CONCLUSION: The 3 X-STR multiplex system is relatively abundant in polymorphic information for forensic identification and paternity testing. |
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