血清幽门螺杆菌CagA蛋白抗体滴度与消化性溃疡的关系

目的　通过比较消化性溃疡和慢性胃炎病人血清中CagA抗体阳性的流行情况 ,探讨消化性溃疡和血清幽门螺杆菌CagA蛋白抗体滴度之间的关系。方法　采用病例对照研究设计方案。研究对象来源于中山医院门诊因中上腹痛或腹胀进行胃镜检查的人群。自 1998年 10月 -1998年 12月共收集胃溃疡病例 2 6例 ,十二指肠球部溃疡 2 7例 ,对照组为同期胃镜和病理证实慢性浅表性胃炎的病例 6 5例。Hp感染的判断采用快速尿素酶和病理Giemsa相结合的方法 ,二者均阳性定为阳性。采用ELISA方法检测HpCagA抗体。结果　总的HpCagA抗体的阳性率为 6 2 5 % ,在胃溃疡组为 76 9% ( 2 0 / 2 6 ) ,在十二指肠球部溃疡组为 88 9% ( 2 4/ 2 7) ,在慢性胃炎组为 49 3 % ( 3 2 / 6 5 ) ,胃溃疡组和十二指肠球部溃疡组均明显高于慢性胃炎组 ,差异有显著性。结论　本研究提示CagA蛋白阳性的幽门螺杆菌菌株在消化性溃疡的发病中起一定作用。

Relationship between the serum titers of antibody to CagA of Hp and peptic ulcer

Aim To evaluate the relationship between Helicobacter pylori(Hp) with CagA protein and peptic ulcer. Methods A Case control study was conducted. Fifty three cases (gastric ulcer 26 and duodenal ulcer 27) and 65 control with chronic gastritis were selected in Zhong Shan Hospital from Oct. to Dec. 1998. All cases were examined by gastro endoscopy and Hp status were edtermined by Urease and Pathologic Giemsa. The enzyme linked immunosorbent assay (ELISA) was used to determine the status of anti CagA IgG.Results Seropositivity of Anti CagA IgG was significantly higher in gastric ulcer (20/26,76.9%) and duodenal ulcer (24/27,88.9%) than that in chronic gastritis (32/65,49.3%). Conclusion The results suggest that Hp with CagA protein may contribute to peptic ulcer.