| 苦碟子注射液中果糖、葡萄糖和蔗糖的含量测定 |
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| 引用本文： | 张红霞,张加余,刘颖,董鲁艳,尹佩华,卢建秋. 苦碟子注射液中果糖、葡萄糖和蔗糖的含量测定[J]. 中南药学, 2014, 0(4): 366-369 |
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| 作者姓名： | 张红霞 张加余 刘颖 董鲁艳 尹佩华 卢建秋 |
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| 作者单位： | [1]北京中医药大学巾药学院,北京100102; [2]北京中医药大学科研实验中心,北京100029 |
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| 基金项目： | 国家“973”计划资助项目（No.2012CB518406）;在校研究生自主课题资助项目（No.5321/0100604229） |
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| 摘 要： | 目的采用高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定苦碟子注射液中果糖、葡萄糖和蔗糖的含量。方法采用Prevail Carbohydrate ES糖分析色谱柱(4.6 mm×250 mm,5μm),乙腈(B)-0.1%乙酸水(A)为流动相,线性梯度洗脱,流速为1.0 mL·min-1,柱温为25℃;ELSD检测器:漂移管温度为80℃,雾化气(空气)流速为1.8 L·min-1,测定苦碟子注射液中果糖、葡萄糖和蔗糖含量。结果果糖、葡萄糖和蔗糖在色谱柱上分离效果良好,其平均加样回收率分别为96.7%(RSD=0.70%)、96.6%(RSD=1.3%)、98.6%(RSD=2.9%)。结论该方法简便、稳定、重复性好,可用于苦碟子注射液中果糖、葡萄糖和蔗糖测定,并为其质量控制提供理论依据。
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| 关 键 词： | 苦碟子注射液 高效液相色谱-蒸发光散射检测法 果糖 葡萄糖 蔗糖 |
Content determination of fructose,glucose and sucrose in Kudiezi injection by HPLC-ELSD |
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| Affiliation: | ZHANG Hong-xia, ZHANG Jia-yu, LIU Ying, DONG Lu-yan, YIN Pei-hua, LU Jian-qiu. (1. School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102; 2. Center of Scientific Experiment, Beijing University of Chinese Medicine, Beijing 100029) |
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| Abstract: | Objective To establish an HPLC-ELSD method to determine the content of fructose, glucose and sucrose in Kudiezi injection. Methods Chromatographic separation was performed on a Prevail Carbohydrate ES column with the mobile phase of acetonitrile（B）-0.1% acetic acid（A） at a fl ow rate of 1.0 mL · min^- 1 with gradient elution. The column temperature was 25 ℃; the ELSD drift tube temperature was 80 ℃ and the gas fl ow was 1.8 L · min^- 1. Results Good separation and linear range were obtained. The average recoveries of fructose, glucose and sucrose were 96.7%（RSD = 0.70%）, 96.6%（RSD = 1.3%）, and 98.61%（RSD = 2.9%）, respectively. Conclusion The established method is simple, reliable and accurate. It can be used to determine the saccharide components in Kudiezi injection, and provide theoretical basis for its quality control. |
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| Keywords: | Kudiezi injection HPLC-ELSD fructose glucose sucrose |
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