樟脑胺氯乙酸铂对L1210白血病细胞生物大分子的影响

樟脑胺氯乙酸铂(camphoramine chloroacetic platinum,CCP)是一新的抗癌铂类化合物。本文报道其对小鼠L₁₂₁₀白血病细胞DNA,RNA和蛋白质含量及合成代谢的影响,结果表明、CCP可使细胞内DNA,RNA及蛋白质含量有不同程度的下降,并可明显抑制DNA的合成。采用同位素、荧光探针技术初步分析讨论了CCP对DNA的作用方式。结果提示CCP可能是DNA模板损伤型药物,与DNA发生共价及非共价结合。

EFFECT OF CAMPHORAMINE CHLOROACETIC PLATINUM (CCP) ONBIOMACROMOLECULES IN L1210 LEUKEMIC CELL

Camphoramine Chloroacetic Platinum (CCP) is a new anticancer platinum complex. In the present paper, the effects of CCP on contents and biosynthesi of DNA, RNA and proteins of L₁₂₁₀ leukemic cell in DBA/2 mice and the mode of action of CCP on DNA template by using isotope and fluoresent probe techniques are reported. Preliminary results revealed that CCP decrease the contents of DNA, RNA and proteins of L₁₂₁₀ leukemic cell in vivo and in vitro and has remarkable inhibition on biosynthesis of DNA in L₁₂₁₀ cell. The action of CCP may be to damage the DNA template and bind to DNA covalently and noncovalently.