| ATP对髓袢升支粗段管周膜50pS钾通道的调节 |
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| 引用本文： | 张成标,杨雷,谷瑞民. ATP对髓袢升支粗段管周膜50pS钾通道的调节[J]. 中国药理学通报, 2010, 26(10) |
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| 作者姓名： | 张成标 杨雷 谷瑞民 |
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| 作者单位： | 1. 徐州医学院麻醉学重点实验室,江苏,徐州,221002
2. 哈尔滨医科大学药理学教研室,黑龙江,哈尔滨,150086 |
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| 基金项目： | 国家自然科学基金资助项目，徐州医学院院长专项科研基金资助项目 |
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| 摘 要： | 目的研究ATP对肾脏髓袢升支粗段管周膜钾通道活性的调控作用。方法应用单通道膜片钳技术记录管周膜钾通道电流,观察ATP作用前后钾通道开放概率的变化。结果在内面向外式记录模式下,1.0mmol·L-1的ATP对管周膜50pS钾通道有抑制作用,通道开放概率从加药前的0.46±0.28降至0.02±0.04;ATP对50pS钾通道的抑制作用具有剂量依赖性。结论髓袢升支粗段管周膜钾通道的活性受细胞内ATP的调控。
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| 关 键 词： | 钾通道 ATP 膜片钳 钠-钾泵 髓袢升支粗段 肾脏 |
Effect of ATP on activity of 50 pS K+ channel in basolateral membrane of the thick ascending limb |
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| ZHANG Cheng-biao,YANG Lei,GU Rui-min. Effect of ATP on activity of 50 pS K+ channel in basolateral membrane of the thick ascending limb[J]. Chinese Pharmacological Bulletin, 2010, 26(10) |
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| Authors: | ZHANG Cheng-biao YANG Lei GU Rui-min |
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| Abstract: | Aim To investigate the effect of ATP on the basolateral K + channels in the thick ascending limb(TAL) of rat kidney.Methods The single channel patch-clamp technique was used to record the basolateral K + channel current in the TAL,and channel open probability was calculated before and after the addition of ATP.Results 1mmol·L -1 ATP inhibited 50 pS K + channels in basolateral membrane of TAL in insideout patches,and channel open probability was decreased from 0.46±0.28 to 0.02±0.04 after addition of ATP.The inhibitory effect of ATP on 50 pS K + channels was dose-dependent.Conclusion The activity of K + channel in basolateral membrane of TAL is regulated by cellular ATP. |
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| Keywords: | ATP |
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