激光辅助孵化对冻融卵裂期胚胎和囊胚移植妊娠结局的影响

目的：探讨激光辅助孵化（LAH）对玻璃化冻融卵裂期胚胎和囊胚移植妊娠结局的影响。方法：回顾性分析565个玻璃化冻融移植周期，根据是否行LAH，将426个卵裂期胚胎移植周期分为AH组和非AH组，139个囊胚移植周期分为AH1组和非AH1组。比较患者一般情况和妊娠结局。结果：卵裂期胚胎AH组和非AH组患者的临床妊娠率、胚胎种植率、多胎率、流产率、异位妊娠率比较差异均无统计学意义（P＞0.05）。囊胚AH1组的胚胎种植率和多胎率高于非AH1组（P＜0.05），2组的临床妊娠率、流产率和异位妊娠率差异无统计学意义（P＞0.05）。结论：LAH可提高玻璃化冻融囊胚移植的胚胎种植率和多胎率，但并不能提高玻璃化冻融卵裂期胚胎移植的临床妊娠率和胚胎种植率。

Effect of Laser-assisted Hatching on Clinical Outcomes of Frozen-thawed Embryo Transfer at Cleavage Stage and Blastocyst

Objective: To investigate the effects of laser-assisted hatching （LAH） on clinical outcomes of vitrified-warmed embryo transfer at the cleavage stage and blastocyst. Methods：565 vitrification-frozen embryo transfer（FET） cycles were retrospectively studied. According to whether the AH was performed, 426 cleavage stage embryo transfer cycles were divided into two groups: AH group and non-AH group; 139 blastocyst transfer cycles were divided into two groups: AH1 group and non-AH1 group. The general condition and pregnancy outcome were compared. Results：There were not significant differences in clinical pregnancy rate, implantation rate, multi-pregnancy rate, miscarriage rate and ectopic pregnancy rate between the AH group and the non-AH group （P＞0.05）. The implantation rate and multi-pregnancy rate in the AH1 group were significantly higher than those in the non-AH1 group （P＜0.05）. There were not significant differences in the rates of clinical pregnancy, miscarriage and ectopic pregnancy between the AH1 group and the non-AH1 group （P＞0.05）. Conclusions：LAH can increase the rates of implantation and multi-pregnancy in vitrified-warmed blastocyst transfer, but it does not increase the clinical pregnancy and implantation rates of vitrified-warmed embryos transfer at the cleavage stage.