| 尿酸通过诱导线粒体钙稳态失衡介导内皮细胞炎症反应 |
| |
| 引用本文： | 齐卡,冯哲,洪权,傅博,崔少远,陈香美,吴镝. 尿酸通过诱导线粒体钙稳态失衡介导内皮细胞炎症反应[J]. 山东医药, 2010, 50(20): 15-18 |
| |
| 作者姓名： | 齐卡 冯哲 洪权 傅博 崔少远 陈香美 吴镝 |
| |
| 作者单位： | 中国人民解放军总医院,北京,100853 |
| |
| 基金项目： | 国家自然科学基金资助项目，国家重点基础研究发展规划"973"项目 |
| |
| 摘 要： | 目的探讨线粒体钙稳态失衡在高尿酸（UA）导致的血管内皮细胞炎症中的病理生理机制。方法采用Fluo-3 AM及Rhod-2 AM分别对UA刺激后的人脐静脉内皮细胞（HUVEC-C）进行胞质钙离子浓度（[Ca2＋]i）和线粒体钙离子浓度（[Ca2＋]mito）的特异性染色;RT-PCR法检测其C反应蛋白（CRP）和细胞间黏附分子1（ICAM-1）的mRNA表达变化;Western blot检测ICAM-1的蛋白变化;ELISA法检测其培养液上清白细胞介素6（IL-6）的释放变化情况。结果HUVEC-C经600μmol/L的UA刺激后,其[Ca2＋]i未见明显变化,而[Ca2＋]mito呈波浪式升高（P〈0.05）,并在24 h内保持较高的状态;CRP、ICAM-1、IL-6均在UA刺激后升高（P〈0.05）。结论UA可导致内皮细胞炎症反应,[Ca2＋]mito的升高与之相关,提示线粒体钙稳态失衡在UA导致的内皮炎症反应中起一定的介导作用。
|
| 关 键 词： | 尿酸 线粒体 钙 内皮细胞 炎症 |
Mitochondrial calcium homeostasis imbalance was associated with uric acid induced endothelial inflammation |
| |
| QI Ka,FENG Zhe,HONG Quan,FU Bo,CUI Shao-yuan,CHEN Xiang-mei,WU Di. Mitochondrial calcium homeostasis imbalance was associated with uric acid induced endothelial inflammation[J]. Shandong Medical Journal, 2010, 50(20): 15-18 |
| |
| Authors: | QI Ka FENG Zhe HONG Quan FU Bo CUI Shao-yuan CHEN Xiang-mei WU Di |
| |
| Affiliation: | QI Ka,FENG Zhe,HONG Quan,FU Bo,CUI Shao-yuan,CHEN Xiang-mei,WU Di(The General Hospital of the People's Liberation Army,Beijing 100853,P.R.China) |
| |
| Abstract: | Objective To evaluate the characteristics and mechanism of mitochondrial calcium homeostasis imbalance in vascular endothelial cells inflammation induced by uric acid.Methods Fluo-3 AM and Rhod-2 AM were employed to measure the introcellular calcium concentration(i) and mitochondrial calcium concentration(mito) of human umbilical vein endothelial cells(HUVEC-C) after stimulation of UA,respectively.RT-PCR was used to detected C-reactive protein(CRP) and the intercellular adhesion molecule-1(ICAM-1) levels of... |
| |
| Keywords: | uric acid mitochondria calcium endothelial cells inflammation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
