用均匀设计方法优化Sertoli-germ细胞培养条件的研究

采用均匀设计方法对Ｓｅｒｔｏｌｉｇｅｒｍ细胞共培养条件进行优化。结果显示，所观察的三个因素（３或６个水平）中，培养温度和ｐＨ值对生精细胞脱落的影响最为明显，而小牛血清浓度的影响较少。回归方程提示该培养系统的适宜条件，逐步回归分析剔除了小牛血清浓度的因素，说明不必加入过多小牛血清。此外，用所获回归方程对指标值进行回算，结果提示，以脱落生精细胞数作为培养效果的观察指标比较稳定，实验误差较小

Optimization of the Condition of Sertoli germ Cell Co Culture by Uniform Design

Uniform design,an advanced methematical method was applicated to optimize Sertoli germ cell co culture condition in the paper.Three factors:pH of the medium cultural temperature,and concentration of foetal bovine serum.with 3 or 6 levels,were investigated in the co culture system.Detached germ cells in the medium under the different culture condition were counted respectively as a study index,after the enzymologically scattered cells were cultured for 24 hours in a humidified 5% CO 2/95% air.The results indicate that the temperature and pH of medium exert the greatest effects on detachment of germ cells,and a lower culture temperature and some higher pH in the medium should be the parts of optimal culture condition for the co culture system.