| LC/MSn鉴定咖啡酸在大鼠体内的代谢产物 |
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| 引用本文： | 苏美英,周婷婷,周茂金.LC/MSn鉴定咖啡酸在大鼠体内的代谢产物[J].中国现代应用药学,2009,26(6):501-505. |
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| 作者姓名： | 苏美英 周婷婷 周茂金 |
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| 作者单位： | 山东省泰安市中心医院，山东 泰安 271000 |
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| 摘 要： | 目的研究咖啡酸（CA）在大鼠体内的代谢产物。方法大鼠灌胃（50mg·kg^-1）给予CA后采集0～4h尿样,用电喷雾离子阱多级质谱法对CA在大鼠体内的代谢产物进行分析。结果大鼠灌胃给予CA后,在体内可测到2个原形药的甲基化代谢物、2个原形药的单葡萄糖醛酸结合物、1个原形药的双葡萄糖醛酸结合物、2个原形药的单硫酸结合物、2个甲基化物的葡萄糖醛酸结合物和2个甲基化物的硫酸结合物。结论CA在大鼠体内广泛代谢,其代谢物的结构有待于进一步分析后确证。
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| 关 键 词： | 咖啡酸 电喷雾离子阱多级质谱法 代谢物 |
| 收稿时间： | 1/1/1900 12:00:00 AM |
The Characterization of the Metabolites of Caffeic Acid in Rats by LC/MSn |
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| SU Meiying,ZHOU Tingting,ZHOU Maojin.The Characterization of the Metabolites of Caffeic Acid in Rats by LC/MSn[J].The Chinese Journal of Modern Applied Pharmacy,2009,26(6):501-505. |
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| Authors: | SU Meiying ZHOU Tingting ZHOU Maojin |
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| Institution: | Taian Central Hospital, Taian 271000,China |
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| Abstract: | OBJECTIVE To investigate the metabolites of caffeic acid (CA) in rats. METHODS After an oral administration of CA (50 mg·kg-1) to Wistar rats, 0-4 h rats urine was collected, and metabolites of CA in rats was determined by liquid chromatography tandem multi-stage mass spectrometry (LC/MSn). RESULTS The results revealed that two methylated derivatives (ferulic and isoferulic acid) of CA, two CA-glucuronides, two CA-sulfates, one CA-di-glucuronide, two methylated CA-glucuronides, two methylated CA-sulfates were found in rats urine. CONCLUSION CA was extensively metabolized in rats and structures of the metabolites will be further confirmed. |
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| Keywords: | HPLC Hypericum japonicum Thunb quercitrin content determination |
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