甲状旁腺激素对成人成骨细胞护骨素和护骨素配体及其相关因子的调节

目的：探讨甲状旁腺激素（PTH）调节骨吸收作用的途径和机制。方法：观察PTH对人成骨细胞(HOB)表达护骨素(OPG)、护骨素配体(OPGL)及其相关因子，如肿瘤坏死因子相关性凋亡诱导配体(TRAIL)、巨噬细胞集落刺激因子（M-CSF）的影响，用RT-PCR法检测OPG，OPGL，TRAIL及M-CSF基因表达情况，用Western blotting分析法检测OPG和OPGL蛋白表达情况。结果：PTH下调HOB细胞中OPG的表达，上调OPGL，M-CSF及TRAIL的表达。结论：PTH通过降低成骨细胞中OPG与OPGL的比值，增加M-CSF和TRAIL的表达，促进破骨细胞生成、活化，促进骨吸收。

Effects of parathyroid hormone on osteoprotegerin expression and osteoprotegerin ligand and their related cytokines in human osteoblasts

Objective To investigate the pathway and mechanism of parathyroid hormone (PTH) in regulating bone resorption.Methods We observed the regulative effects of PTH on the expression of osteoprotegerin (OPG) and osteoprotegerin ligand (OPGL) and their related cytokines (M-CSF and TRAIL) in human osteoblasts (HOBs).The expressions of OPG,OPGL,M-CSF, and TRAIL mRNA were determined by RT-PCR. The expressions of OPG and OPGL proteins were measured by Western blotting. Results PTH down-regulated the expression of OPG but up-regulated the expressions of OPGL,M-CSF and TRAIL in HOBs.Conclusion PTH can promote the bone resorption by decreasing the expression of OPG ,increasing the expressions of some bone-resorbing cytokines such as OPGL, M-CSF, and TRAIL in osteoblasts,and then stimulating the osteoclast differentiation and activity.