Gangliosides modulate glutamate receptor binding in rat brain synaptic plasma membranes |
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| Authors: | M Hollmann W Seifert |
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| Affiliation: | 1. School of Civil Engineering and Mechanics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, PR China;2. School of Urban Construction, Yangtze University, Jingzhou, Hubei 434000, PR China;1. Interventional Cardiology, Division of Cardiology, Massachusetts General Hospital, Harvard Medical School, Boston;2. Cardiovascular Division, University of Miami, Miller School of Medicine, Fla;3. Department of Pediatrics, Texas Tech University Health Sciences Center, Lubbock;4. Division of Cardiology, Henry Ford Hospital, Detroit, Mich;1. Instituto de Neurobiología, Campus Juriquilla, Universidad Nacional Autónoma de México, Mexico;2. Departamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, Unidad Lerma, Mexico;3. Departamento de Farmacología y Toxocología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Mexico;4. Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo León, Mexico;5. Instituto de Fisiología Celular, División de Neurociencias Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico;1. Department of Critical Care Medicine, Shanghai Tenth People’s Hospital, Tongji University, Shanghai, China;2. Center for Diffcult and Complicated Abdominal Surgery, Shanghai Tenth People’s Hospital, Tongji University, Shanghai, China |
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| Abstract: | The influence of gangliosides on the binding of L-[3H]glutamate (Glu) to its receptor(s) on isolated rat brain synaptic plasma membranes was investigated in two different buffer systems using an in vitro filter binding assay. It was found that the tested pure monosialogangliosides GM1 and GM2 as well as the polysialogangliosides GT1b and GD1a enhanced binding by up to about 100%, but only in the presence of 5 mM calcium. The binding site involved was sensitive to quisqualate but insensitive to N-methyl-D-aspartate and kainate. GM1 did exert its stimulating effect by increasing the number of binding sites on the membranes whereas the receptor's affinity for L-Glu was unchanged. Regional differences in rat brain were found: while the hippocampus and cortex exhibited significant Ca/GM1-induced stimulation of Glu binding, the cerebellum was unaffected. Our results suggest modulation of at least one subtype of the Glu receptor by gangliosides. |
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