过量氟对大鼠下切牙发育过程中釉蛋白表达的影响

目的 研究过量氟对大鼠下切牙发育过程中釉蛋白表达的影响。方法 选择20只Wistar大鼠，随机分成实验组和对照组。饲养8周后处死，利用HE染色和免疫组织化学染色的方法观察过量氟对大鼠成釉细胞形态及釉蛋白表达的影响。结果 实验组大鼠下切牙成釉细胞由原有的高柱状结构变矮，细胞排列成多层，釉基质形成混乱，成釉细胞和成牙本质细胞中釉蛋白表达明显低于对照组，其差异有统计学意义（P<0.01）。结论 过量氟可抑制釉蛋白表达，影响釉质发育。

Effect of Overdose Fluoride on the Expression of Enamelin in Rat Mandibular Incisor

OBJECTIVE: To observe the effect of overdose fluoride on the expression of enamelin in rat mandibular incisor. METHODS: Twenty Wistar rats were divided randomly into two groups. Animals were maintained in standard environment with free access to food and distilled water (control group) or water added with 100 mg/L F-(experimental group). The rats were killed in the eighth week. HE staining was used to observe the morphology of ameloblasts. Immunohistochemical staining was adopted to study the expressions of enamelin in rat incisor. RESULTS: The ameloblasts of the treated rat were arranged in multi-layer. The ameloblasts in group II were thinner than those in group I. The structure of enamel matrix was in disorder. The expressions of enamelin in ameloblasts and odontoblasts were obviously inhibited in group II (P < 0.01). CONCLUSION: The overdose fluoride inhibits the secretion of enamelin and leads to the abnormal development of enamel matrix.