PTEN及nm23-H1蛋白表达在非小细胞肺癌转移中的意义

背景与目的PTEN及nm23-H1基因均被证实是肿瘤转移的抑制基因,目前大多数研究都停留在基础研究上,本研究通过检测PTEN及nm23-H1蛋白在非小细胞肺癌(NSCLC)中的表达,以探讨它们的临床意义及相互关系。方法应用免疫组织化学法检测60例非小细胞肺癌组织中PTEN及nm23-H1基因蛋白的表达。结果NSCLC无淋巴结转移组PTEN蛋白阳性表达率为79.31%,高于NSCLC伴有淋巴结转移组41.94%,两者差异有统计学意义(P<0.01);无淋巴结转移组nm23-H1蛋白阳性表达率为82.76%,高于NSCLC伴有淋巴结转移组45.16%,两者差异有统计学意义(P<0.01)。PTEN和nm23-H1蛋白表达在NSCLC中一致性较好(Kappa=0.4366,Z=3.3905,P<0.01),两基因可能有协同作用。结论PTEN及nm23-H1蛋白表达均与NSCLC的淋巴结转移有关,PTEN和nm23-H1蛋白均可作为预测肿瘤转移的重要指标,对两种蛋白进行免疫组织化学联合检测,可能更有利于肺癌预测淋巴结转移及预后。

Expression and significance of PTEN and nm23-H1 in the metastasis of non-small cell lung cancer

Background and objective It has been proven that PTEN and nm23-H1 genes were the tumor metastasis suppressor genes.Up to now,most of studies are aim at basic research.The aim of this study is to explore the level of expression of PTEN and nm23-H1 protein in non-small cell lung cancer and their relationship to the clinical significance of non-small cell lung cancer.Methods An immunohistochemical method was used to detect the expression of PTEN and nm23-H1 protein in 60 cases of non-small cell lung cancer.Results In the groups with and without lymph nodes metastasis,the positive rates of PTEN protein expression were 41.94% and 79.31% respectively.The difference between them was significant(P<0.01).The rates of positive expression of nm23-H1 protein were 45.16% and 82.76% respectively.The difference between them also was significant(P<0.01).The expression of PTEN was relatively consistent with that of nm23-H1(Kappa=0.436 6,Z=3.390 5,P<0.01).Conclusion The expressions of PTEN and nm23-H1 in NSCLC were closely related to lymph nodes metastasis.Combining detection of these two proteins might be more helpful for evaluating lymph nodes metastasis and predicting the prognosis.